Effect of Electrical Stimulation to Prevent Muscle Atrophy on Morphologic and Histologic Properties of Hindlimb Suspended Rat Hindlimb Muscles
Effect of Electrical Stimulation to Prevent Muscle Atrophy on Morphologic and Histologic Properties of Hindlimb Suspended Rat Hindlimb Muscles
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DOI：
10.1097/phm.0b013e31818e02d6
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发表时间：
2009-09-01
影响因子：
3
通讯作者：
Murakami, Shinji
中科院分区：
文献类型：
--
作者：
Boonyarom, Onuma;Kozuka, Naoki;Murakami, Shinji
目的：确定电刺激（ES）预防肌肉萎缩的形态学和组织学效应，并探讨不同刺激频率下的 ES 对预防不同肌纤维类型萎缩的效果。设计：后肢悬吊（HS）加 ES 组大鼠每隔一天接受 20 赫兹和 30 赫兹刺激（ES 1、ES2），持续 2 周。结果：比目鱼肌的肌肉重量、肌纤维横截面积和数量HS和HS+ES2组中I型肌纤维的数量显着减少，而HS+ES1组中I型肌纤维的数量保持不变。这表明，基于组织学特性，20 Hz 的 ES 可以抑制肌肉萎缩并保留肌纤维类型比例。在趾长伸肌中，HS组的肌肉重量、肌纤维横截面积和II型肌纤维数量显着减少，而HS+ES组则保持不变。这表明 20 Hz 或 30 Hz 的 ES 可以抑制肌肉萎缩，并根据组织学特性保留肌纤维类型比例。 30 Hz 的 ES 对维持趾长伸肌也有积极作用。 结论：这些结果表明，在不活动期间对肌肉进行短时间的低强度、低刺激频率 (20 Hz) ES 可以维持慢肌纤维（比目鱼肌）形态和组织学特性的变化。在不活动期间对肌肉进行短时间的低强度、高刺激频率 (30 Hz) ES 可以维持快肌纤维（趾长伸肌）形态和组织学特性的变化。
Objective: To determine the morphologic and histologic effects of electrical stimulation (ES) used to prevent muscle atrophy, and to investigate the effects of ES at different stimulation frequencies in preventing atrophy in different muscle fiber types.Design: Rats in the hindlimb suspended (HS) plus ES group were subjected to 20- and 30-Hz stimulation (ES 1, ES2) every other day for 2 wks.Results: In soleus, the muscle weight, muscle fiber cross-sectional area, and the number of type I muscle fibers were significantly decreased in the HS and HS + ES2 groups, whereas they were maintained in the HS + ES1 group. This indicated that ES at 20 Hz could suppress muscle atrophy and retain muscle fiber type proportions, based on histologic properties. In extensor digitorum longus, the muscle weight, muscle fiber cross-sectional area, and the number of type II muscle fibers were significantly decreased in the HS group, whereas they were maintained in the HS + ES groups. This indicated that ES at either 20 Hz or 30 Hz could suppress muscle atrophy, and retain muscle fiber type proportions, based on histologic properties. ES at 30 Hz also had positive effects in maintaining the extensor digitorum longus muscle.Conclusions: These results suggest that short periods of low-intensity, low-stimulation frequency (20 Hz) ES of muscle during periods of inactivity could maintain changes in both morphologic and histologic properties of the slow-twitch muscle fibers (soleus). Short periods of low-intensity, high-stimulation frequency (30 Hz) ES of muscle during periods of inactivity could maintain changes in both morphologic and histologic properties of the fast-twitch muscle fibers (extensor digitorum longus).