Artificial bio-nanomachines based on protein needles derived from bacteriophage T4
Artificial bio-nanomachines based on protein needles derived from bacteriophage T4
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DOI：
10.1007/s12551-017-0336-9
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发表时间：
2018-04
影响因子：
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通讯作者：
H. Inaba;T. Ueno
中科院分区：
文献类型：
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作者：
H. Inaba;T. Ueno
噬菌体T4是一种天然的生物纳米机器，通过特定的三维蛋白质结构的协同运动来实现对宿主细胞的有效感染。蛋白质结构与其动力学功能之间的关系最近已被阐明。在本文中，我们总结了基于这些最新进展的噬菌体T4的组成蛋白的纳米机器制造的设计原则。我们专注于被称为gp 5的蛋白质针，它位于T4噬菌体收缩尾末端基板的中心。这种蛋白质针在直接穿刺宿主细胞中起着关键作用，并且分析显示它包含用于在其他已知注射系统（例如T6 SS）中进行细胞穿刺的常见基序。我们基于gp 5的β-螺旋结构域的人工针保留了穿透细胞的能力，并且可以被工程化以将各种货物递送到活细胞中。因此，噬菌体T4和其他天然纳米机器的独特组分具有巨大的潜力，可用作分子支架，以制造新的生物纳米机器。
Bacteriophage T4 is a natural bio-nanomachine which achieves efficient infection of host cells via cooperative motion of specific three-dimensional protein architectures. The relationships between the protein structures and their dynamic functions have recently been clarified. In this review we summarize the design principles for fabrication of nanomachines using the component proteins of bacteriophage T4 based on these recent advances. We focus on the protein needle known as gp5, which is located at the center of the baseplate at the end of the contractile tail of bacteriophage T4. This protein needle plays a critical role in directly puncturing host cells, and analysis has revealed that it contains a common motif used for cell puncture in other known injection systems, such as T6SS. Our artificial needle based on the β-helical domain of gp5 retains the ability to penetrate cells and can be engineered to deliver various cargos into living cells. Thus, the unique components of bacteriophage T4 and other natural nanomachines have great potential for use as molecular scaffolds in efforts to fabricate new bio-nanomachines.