Fungi associated with chimney and sulfide samples from a South Mid-Atlantic Ridge hydrothermal site: Distribution, diversity and abundance
Fungi associated with chimney and sulfide samples from a South Mid-Atlantic Ridge hydrothermal site: Distribution, diversity and abundance
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与南大西洋中脊热液地点的烟囱和硫化物样本相关的真菌：分布、多样性和丰度
DOI：
10.1016/j.dsr.2017.03.004
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Luo Zhu Hua
中科院分区：
文献类型：
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作者：
Xu Wei;Guo Shuangshuang;Pang Ka Lai;Luo Zhu Hua
众所周知，深海热液喷口生态系统支持着极其多样化的微生物群落，从化学自养原核生物到异养原核生物和微真核生物。虽然真菌通常被认为是环境中各种微生物群落的重要组成部分，但对深海热液喷口生态系统中此类微生物的物种丰富度和丰富度知之甚少。在这项研究中，采用依赖于培养和非依赖于培养的测序相结合的方法，调查了位于南大西洋中大西洋脊的深海热液喷口地点的真菌分布和多样性。序列分析表明，真菌群落主要由子囊菌门和担子菌门的成员组成。确定了几个新的系统类型(65个真菌OTU中的28个和19个可培养的真菌系统中的2个)，导致了这个未被研究的栖息地真菌的真正未知的多样性。烟囱样品中的真菌群落结构与3个硫化物样品中的不同。定量聚合酶链式反应结果表明，真菌LSU rRNA基因拷贝数在5.88×105~6.77×106拷贝/克岩石(湿重)之间，子囊菌明显比担子菌门丰富2~3个数量级。我们的发现为深海热液喷口生态系统中真菌的多样性和丰富性提供了新的见解，增加了我们对深海环境中真菌多样性的认识和理解。
Deep-sea hydrothermal vent ecosystems are known to support remarkably diverse microbial communities, ranging from chemoautotrophic prokaryotes to heterotrophic prokaryotes and microeukaryotes. While fungi have generally been identified as an important component of various microbial communities in the environment, little is known about the species richness and abundance of such microorganisms in deep-sea hydrothermal vent ecosystems. In this study, a combined culture-dependent and culture-independent sequencebased approach was used to investigate fungal distribution and diversity at a deep-sea hydrothermal vent site located at the Mid-Atlantic Ridge of the South Atlantic Ocean. Sequence analyses showed that the fungal community was dominated by members of the Ascomycota and the Basidiomycota. Several new phylotypes (28 of 65 total fungal OTUs and 2 of 19 culturable fungal phylotypes) were identified, contributing to the literally unknown diversity of fungi in this understudied habitat. The fungal community structures in the chimney samples were distinct from those in three sulfide samples. The qPCR results revealed that fungal LSU rRNA gene copy numbers ranged from 5.88×105 to 6.77×106 copies/gram rock (wet weight), and the Ascomycota was significantly more abundant 2–3 orders) than the Basidiomycota. Our findings provide new insights into the diversity and abundance of fungi in deep-sea hydrothermal vent ecosystems, which increases our knowledge and understanding of the fungal diversity in deep-sea environments.