New Nematotoxic Indoloditerpenoid Produced by Gymnoascus reessii za-130
New Nematotoxic Indoloditerpenoid Produced by Gymnoascus reessii za-130
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DOI：
10.1021/acs.jafc.6b04353
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发表时间：
2017-04-19
影响因子：
6.1
通讯作者：
Liu, Wei-cheng
中科院分区：
文献类型：
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作者：
Liu, Ting;Meyer, Susan L. F.;Liu, Wei-cheng
从侵染南方根结线虫的番茄根际中分离到一株真菌菌株Gymnoascus reessii za-130，对其化学成分进行了研究，分离鉴定了一种新的吲哚内酯类代谢产物，命名为裸子藻乙酸酯。其结构经一维、二维核磁共振和MS等波谱方法确证。醋酸酯裸露醇对南方根结线虫第二期幼虫(J2)的活力有较强的抑制作用，36 mU g/m L作用24 h可使J2 100%瘫痪(EC50=47.5 mU g/m L)。醋酸酯裸露醇对南方根结线虫卵孵化的抑制率为90%，作用7d后的抑制率为133µg/mL。与对照相比，2~200mgg/mL裸子菌/kg土壤处理显著减少了根瘤和根部J2的数量(p=0.05)，线虫抑制率随着裸子菌醋酸酯浓度的增加而增加。本研究证实了裸子植物醋酸酯对线虫的毒性，并表明它可能是南方根结线虫综合治理的潜在生物基成分。
Chemical investigation of the fungal strain Gymnoascus reessii za-130, which was previously isolated from the rhizosphere of tomato plants infected by the root-knot nematode Meloidogyne incognita, led to the isolation and identification of a new indoloditerpenoid metabolite designated gymnoascole acetate. Its structure was established by spectroscopic methods including 1D- and 2D-NMR and MS analyses. Gymnoascole acetate demonstrated strong adverse effects on M. incognita second stage juvenile (J2) viability; exposure to 36 mu g/mL for 24 h induced 100% paralysis of J2 (EC50 = 47.5 mu g/mL). Gymnoascole acetate suppressed M. incognita egg hatch relative to controls by > 90% at 133 mu g/mL after 7 days of exposure. The numbers of root galls and J2 in both soil and roots were significantly reduced (p = 0.05) by treatment with 2-200 mu g/mL gymnoascole acetate/kg soil, compared to untreated control plants; nematode suppression increased with gymnoascole acetate concentration. This study demonstrated the nematotoxicity of gymnoascole acetate and indicates that it might be a potential biobased component in integrated management of M. incognita.