Isolation and functional analysis of a pepper lipid transfer protein III (CALTPIII) gene promoter during signaling to pathogen, abiotic and environmental stresses
Isolation and functional analysis of a pepper lipid transfer protein III (CALTPIII) gene promoter during signaling to pathogen, abiotic and environmental stresses
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DOI：
10.1016/j.plantsci.2005.08.010
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发表时间：
2006-02-01
期刊：
影响因子：
5.2
通讯作者：
Hwang, BK
中科院分区：
文献类型：
--
作者：
Jung, HW;Lim, CW;Hwang, BK
从辣椒的基因组DNA中分离出编码碱性脂质转移蛋白的CALTP III基因的翻译起始位点上游1065 bp的启动子片段。在CALTPIII基因的启动子区发现了一些推定的顺式作用元件，包括ERE盒、W盒和MYB核心元件。在农杆菌介导的瞬时表达试验中，烟草叶片感染假单胞菌pv.烟粉虱，以及乙烯、水杨酸、茉莉酸甲酯和H_2O_2处理。然而，完整的启动子没有响应脱落酸，而以前的研究表明，CALTPIII mRNA确实是由阿坝诱导。病原菌和乙烯反应主要受-830和-422 bp之间的缺失的影响。在任何缺失的构建体中丢失水杨酸反应。精细分析表明，病原体反应的关键元件位于-626和-552 bp之间。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
The promoter fragment of 1065 bp upstream from the translation initiation site of the CALTPIII gene encoding a basic lipid transfer protein was isolated from the genomic DNA of Capsicum annuum. Some putative cis-acting elements, including an ERE-box, a W-box, and MYB-core elements were found in the promoter region of the CALTPIII gene. In Agrobacterium-mediated transient expression assay, strong activation of the CALTPIII full promoter region (1065 bp long) occurred in tobacco leaves after infection with Pseudomonas syringae pv. tabaci, and treatments with ethylene, salicylic acid, methyl jasmonate and H2O2. However, the full promoter did not respond to abscisic acid while previous studies have shown that the CALTPIII mRNA is indeed induced by ABA. Pathogen and ethylene responses were mostly affected by the deletion between - 830 and -422 bp. Salicylic acid response was lost in any deleted construct. A fine analysis showed that the crucial elements for pathogen response are located between -626 and -552 bp. (c) 2005 Elsevier Ireland Ltd. All rights reserved.