Metabolic specialization in itaconic acid production: a tale of two fungi.
Metabolic specialization in itaconic acid production: a tale of two fungi.
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DOI：
10.1016/j.copbio.2019.09.014
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发表时间：
2020-04
影响因子：
7.7
通讯作者：
N. Wierckx;G. Agrimi;P. S. Lübeck;Matthias G. Steiger;N. Mira;P. Punt
中科院分区：
文献类型：
--
作者：
N. Wierckx;G. Agrimi;P. S. Lübeck;Matthias G. Steiger;N. Mira;P. Punt
曲霉和黑霉通过非常不同的途径产生衣康酸。转运蛋白在真菌有机酸生产中起着核心作用。最近的发现挑战了长期以来对油酸生产的假设。一些最古老和最成熟的工业生物技术过程涉及真菌生产有机酸。在这些真菌中，代谢物在细胞间的运输及其分泌是一个主要因素。在这篇综述中，我们举例说明了线粒体和质膜转运蛋白在两种非常不同的真菌系统，曲霉和黑穗菌中衣康酸生产的重要性。同源和异源两种转运体的过表达，以及线粒体转运体功能的生化分析表明，这两种真菌通过非常不同的途径产生相同的化合物。这些真菌对衣康酸胁迫的反应方式，特别是在低pH值下，也有所不同，尽管这仍然是一个开放的领域，显然需要进一步的研究。
HighlightsAspergillus and Ustilago produce itaconic acid by very different pathways.Transporters play a central role in fungal organic acid production.Recent discoveries challenge long-standing assumptions in itaconate production.Some of the oldest and most established industrial biotechnology processes involve the fungal production of organic acids. In these fungi, the transport of metabolites between cellular compartments, and their secretion, is a major factor. In this review we exemplify the importance of both mitochondrial and plasma membrane transporters in the case of itaconic acid production in two very different fungal systems, Aspergillus and Ustilago. Homologous and heterologous overexpression of both types of transporters, and biochemical analysis of mitochondrial transporter function, show that these two fungi produce the same compound through very different pathways. The way these fungi respond to itaconate stress, especially at low pH, also differs, although this is still an open field which clearly needs additional research.