Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast.
Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast.
复制标题
ATG11将ATG9募集到脑吞噬体结构中对于萌芽酵母的选择性自噬至关重要。
DOI：
10.1083/jcb.200606084
复制
发表时间：
2006-12-18
影响因子：
7.8
通讯作者：
Klionsky, Daniel J
中科院分区：
文献类型：
--
作者：
He, Congcong;Song, Hui;Yorimitsu, Tomohiro;Monastyrska, Iryna;Yen, Wei-Lien;Legakis, Julie E;Klionsky, Daniel J
自噬是真核细胞在各种应激和发育条件下诱导的一种保守的降解途径。它允许消除溶酶体/液泡中的胞质蛋白和细胞器。在酵母酿酒酵母中，线粒体和前自噬体结构（PAS）之间的完整膜蛋白Atg 9（自噬相关蛋白9）周期，形成的螯合囊泡的成核位点，这表明在自噬过程中为囊泡的形成和/或扩张提供膜的作用。为了更好地了解参与Atg 9循环的机制，我们进行了酵母双杂交筛选，并确定了与Atg 9相互作用的外周膜蛋白Atg 11。我们发现，Atg 11管辖Atg 9循环通过PAS在特定的自噬。我们还表明，肌动蛋白细胞骨架的完整性是必不可少的正确的靶向Atg 11的PAS。我们建议，池Atg 11介导的顺行运输Atg 9 PAS是依赖于肌动蛋白细胞骨架在酵母营养生长。
Autophagy is a conserved degradative pathway that is induced in response to various stress and developmental conditions in eukaryotic cells. It allows the elimination of cytosolic proteins and organelles in the lysosome/vacuole. In the yeast Saccharomyces cerevisiae, the integral membrane protein Atg9 (autophagy-related protein 9) cycles between mitochondria and the preautophagosomal structure (PAS), the nucleating site for formation of the sequestering vesicle, suggesting a role in supplying membrane for vesicle formation and/or expansion during autophagy. To better understand the mechanisms involved in Atg9 cycling, we performed a yeast two-hybrid–based screen and identified a peripheral membrane protein, Atg11, that interacts with Atg9. We show that Atg11 governs Atg9 cycling through the PAS during specific autophagy. We also demonstrate that the integrity of the actin cytoskeleton is essential for correct targeting of Atg11 to the PAS. We propose that a pool of Atg11 mediates the anterograde transport of Atg9 to the PAS that is dependent on the actin cytoskeleton during yeast vegetative growth.