Identification of active and quiescent adipose vascular stromal cells
Identification of active and quiescent adipose vascular stromal cells
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DOI：
10.3109/14653249.2011.627918
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发表时间：
2012-02-01
期刊：
影响因子：
4.5
通讯作者：
Lin, Ching-Shwun
中科院分区：
文献类型：
--
作者：
Lin, Guiting;Xin, Zhongcheng;Lin, Ching-Shwun
背景的目标。最近的研究表明，骨髓、毛囊和肠道中都存在活跃和静止的干细胞。我们试图在脂肪组织中识别活跃的和静止的血管基质细胞（VSC）。方法。为了鉴定活性VSC，将胸腺嘧啶类似物5-乙基-2-脱氧尿苷（EdU）腹腔注射给成年大鼠，并于3天后采集其皮下组织。为了鉴定静止VSC，新生大鼠腹腔注射EdU， 9周后采集皮下组织。采集的脂肪组织检测EdU与VSC标志物CD34、平滑肌标志物SMA、内皮标志物RECA和周细胞标志物CD140b的共定位。结果。在注射EdU后3 d的成年大鼠脂肪组织中，EdU值为28.80 +/- 8.70（平均+/- SD）
Background aims. Recent studies have demonstrated the existence of both active and quiescent stem cells in bone marrow, hair follicle and intestine. We attempted to identify active and quiescent vascular stromal cells (VSC) in adipose tissue. Methods. For identification of active VSC, adult rats were injected intraperitoneally with thymidine analog 5-ethynyl-2-deoxyuridine (EdU) and their subcutaneous tissue harvested 3 days later. For identification of quiescent VSC, newborn rats were injected intraperitoneally with EdU and their subcutaneous tissue harvested 9 weeks later. The harvested adipose tissues were examined for the co-localization of EdU with VSC marker CD34, smooth muscle marker SMA, endothelial marker RECA and pericyte marker CD140b. Results. In adult rat adipose tissues harvested 3 days after EdU injection, there were 28.80 +/- 8.70 (mean +/- SD) EdU