A superfusion system technique for the study of the sites of action of glucocorticoids in the rat hypothalamus-pituitary-adrenal system in vitro. I. Pituitary cell superfusion.
A superfusion system technique for the study of the sites of action of glucocorticoids in the rat hypothalamus-pituitary-adrenal system in vitro. I. Pituitary cell superfusion.
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一种用于体外研究糖皮质激素在大鼠下丘脑-垂体-肾上腺系统中作用位点的灌注系统技术。
DOI：
10.1210/endo-100-4-1143
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发表时间：
1977
期刊：
影响因子：
4.8
通讯作者：
P. G. Smelik
中科院分区：
文献类型：
--
作者：
G. H. Mulder;P. G. Smelik
将大鼠垂体前叶细胞置于灌注柱中，用大鼠下丘脑提取物刺激。ACTH分泌率监测收集每2分钟从柱洗脱的部分。用分散的大鼠肾上腺皮质细胞测定过液中ACTH的含量。细胞刺激后6 ~ 12秒出现ACTH。1/8 ~ 1大鼠下丘脑提取物与ACTH释放量呈线性对数剂量-反应关系。CRF刺激同一柱垂体细胞的重复脉冲导致ACTH的重复，相同的峰值。持续的刺激会导致激素释放达到一个平台期，只要刺激存在，这个平台期就会持续。体外灌注皮质酮（0.2马克杯/毫升）或体内垂体供体动物皮质酮（5mg /100g BW）、皮质醇或地塞米松均可抑制受刺激的ACTH释放，但不能抑制基底ACTH释放。供体动物肾上腺切除术导致ACTH基础和刺激释放增加。
Rat anterior pituitary cells were placed in a superfusion column and were stimulated by rat hypothalamic extract. The rate of ACTH secretion was monitored by collecting fractions eluting from the column every 2 min. Dispersed rat adrenocortical cells were used to determine the amounts of ACTH in the superfusate. ACTH appeared in the medium 6-12 sec after stimulation of the cells. A linear log dose-response relationship existed between 1/8 to 1 rat hypothalamus extract and the amounts of ACTH released. Repeated pulses of CRF stimulation of the same column of pituitary cells resulted in repeated, identical peaks of ACTH. Constant stimulation caused a plateau in hormone release that lasted as long as the stimulus was present. The stimulated, but not the basal ACTH release, could be inhibited by superfusion with corticosterone in vitro (0.2 mug/ml), or by treating the pituitary donor animal in vivo with corticosterone (5 mg/100g BW), cortisol or dexamethasone. Adrenalectomy of the donor animal caused increases in the basal and the stimulated ACTH release.