DEVELOPMENT OF AMPEROMETRIC BIOSENSORS BASED ON THE IMMOBILIZATION OF ENZYMES IN POLYMER-FILMS ELECTROGENERATED FROM A SERIES OF AMPHIPHILIC PYRROLE DERIVATIVES
DEVELOPMENT OF AMPEROMETRIC BIOSENSORS BASED ON THE IMMOBILIZATION OF ENZYMES IN POLYMER-FILMS ELECTROGENERATED FROM A SERIES OF AMPHIPHILIC PYRROLE DERIVATIVES
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DOI：
10.1016/0003-2670(95)00178-3
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发表时间：
1995-07-20
影响因子：
6.2
通讯作者：
MAILLEY, P
中科院分区：
文献类型：
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作者：
COCHEGUERENTE, L;COSNIER, S;MAILLEY, P
一系列的两亲性吡咯烷基铵离子不同的大小，他们的铵头已被用于固定在电极表面的辣根过氧化物酶，半乳糖氧化酶，多酚氧化酶，葡萄糖氧化酶和黄嘌呤氧化酶在聚吡咯膜从吸附的两亲性吡咯-酶的混合物电生成。不同聚合物的酶保留特性已被确定，表明疏水性较低的单体具有最佳的固定化效率。半乳糖，葡萄糖和次黄嘌呤检测进行的电化学分析清楚地表明，生物电极的灵敏度与主体聚合物的渗透性。还检查了葡萄糖氧化酶电极对干扰剂如抗坏血酸盐、尿酸盐和对乙酰氨基酚的选择性，当以生理浓度存在时，这些试剂在一定程度上干扰。
A series of amphiphilic pyrrolyl-alkylammonium ions differing in the size of their ammonium heads has been used for the immobilization at the electrode surface of horseradish peroxidase, galactose oxidase, polyphenol oxidase, glucose oxidase and xanthine oxidase in polypyrrolic films electrogenerated from adsorbed amphiphilic pyrrole-enzyme mixtures. The enzyme retention properties of the different polymers have been determined indicating that the less hydrophobic monomer has the best immobilization efficiency. The electrochemical assays performed for galactose, glucose and hypoxanthine detection show clearly that the bioelectrode sensitivity is related to the permeability of the host polymer. The selectivity of the glucose oxidase electrodes towards interfering agents like ascorbate, urate and acetaminophen has also been examined, these agents interfering to some extent when present at physiological concentrations.