Electrophysiological study of spinothalamic inputs to ventrolateral and adjacent thalamic nuclei of the cat.
Electrophysiological study of spinothalamic inputs to ventrolateral and adjacent thalamic nuclei of the cat.
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猫腹外侧和邻近丘脑核的脊髓丘脑输入的电生理学研究。
DOI：
10.1152/jn.1991.66.3.1033
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发表时间：
1991
影响因子：
2.5
通讯作者：
Jones,EG
中科院分区：
文献类型：
--
作者：
Yen,CT;Honda,CN;Jones,EG
1.本研究采用细胞外和细胞内方法记录猫丘脑腹外侧核及其邻近核团的神经纤维和神经元。记录的单位的感受野进行了分析和测试的单位输入内侧丘系（ML）和脊髓丘脑束（STT）的背柱（DC）和腹外侧索（VLF）在C2-3脊髓水平的电刺激。2.在丘脑中分离出38个STT纤维。其传导速度范围为15至75 m/s（模式36 m/s）。这些纤维中有23个被发现有足够的刺激。17个为低阈值（LT）单位，3个为宽动态范围（WDR）单位，3个为高阈值（HT）单位。3.五个STT纤维轴突内注射。其中三个填充得足够好，可以分析其终端字段。中速STT纤维（传导速度38 m/s）在中央外侧核（CL）中有一个4.3微米的轴突和一个单一的大终末场。另外两种STT纤维较小，直径为2.5和2.3微米，传导速度为15和19米/秒，终末场由腹后外侧核（VPL）边缘的几个小终扣组成。4.在分离的319个神经元中，VL核129个中有14个（10.8%），VPL核或腹后内侧核（VPM）76个中有14个（18.4%），CL核64个中有27个（42.2%），网状核（R）50个中有5个（10%）在潜伏期内对VLF刺激有反应。在所有这些核团中，三个脉冲的序列在驱动VLF反应神经元方面比单个脉冲更有效。VLF反应细胞在VL中广泛分布，在CL中集中于一个病灶，并分布在VPL的边界周围。刺激运动皮层可逆向激活VL区的大部分和CL区的少数神经元。5.突触前反应（STT纤维）对VLF刺激的潜伏期短，从0.8到3.9 ms不等（模式1.6 ms）。尽管如此，很少发现快速反应神经元，这些是6个VPL神经元（2.5 - 4 ms），1个VL神经元（3 ms）和4个CL神经元（3-4 ms）。大多数丘脑神经元对VLF刺激的起始峰电位在6 ~ 8 ms和10-15 ms之间出现两个峰值。（400字处截断摘要）
1. Extracellular and intracellular methods were used to record from fibers and neurons in the ventral lateral (VL) and adjacent nuclei of the cat thalamus. The receptive fields of the recorded units were analyzed and the units tested for inputs from the medial lemniscus (ML) and spinothalamic tract (STT) by electrical stimulation of the dorsal columns (DC) and ventrolateral funiculus (VLF) at the C2-3 spinal level. 2. Thirty-eight STT fibers were isolated in the thalamus. Their conduction velocities ranged from 15 to 75 m/s (mode 36 m/s). Adequate stimuli were found for 23 of these fibers. Seventeen were low-threshold (LT), 3 were wide-dynamic-range (WDR), and 3 were high-threshold (HT) units. 3. Five STT fibers were intra-axonally injected. Three were sufficiently well filled for analysis of their terminal fields. An intermediate-velocity STT fiber (conduction velocity 38 m/s) had a 4.3-microns axon and a single large terminal field in the central lateral nucleus (CL). The other two STT fibers were smaller, with diameters of 2.5 and 2.3 microns, conduction velocities of 15 and 19 m/s, and terminal fields made up of a few small boutons at the borders of the ventral posterior lateral nucleus (VPL). 4. Of 319 neurons isolated, 14 out of 129 (10.8%) in VL, 14 out of 76 (18.4%) in the VPL or ventral posterior medial (VPM) nucleus, 27 out of 64 (42.2%) in the CL nucleus, and 5 out of 50 (10%) in the reticular nucleus (R) responded at latencies less than 50 ms to VLF stimuli. A train of three pulses was more effective in driving VLF-responding neurons in all these nuclei than a single pulse. VLF-responding cells were widely dispersed in VL, concentrated in a focus in CL, and distributed around the borders of VPL. Most of those in VL and a small number in CL could be antidromically activated by stimulation of motor cortex. 5. Latencies of presynaptic responses (STT fibers) to VLF stimulation were short and varied from 0.8 to 3.9 ms (mode 1.6 ms). Despite this, very few fast-responding neurons were found. These were six VPL neurons (2.5 to 4 ms), one VL neuron (3 ms), and four CL neurons (3-4 ms). The initial spike latencies of the majority of thalamic neurons responding to VLF stimulation appeared in two peaks, one between 6 and 8 ms and the other at 10-15 ms.(ABSTRACT TRUNCATED AT 400 WORDS)