THE SMALL GTP-BINDING PROTEIN RHO REGULATES THE ASSEMBLY OF FOCAL ADHESIONS AND ACTIN STRESS FIBERS IN RESPONSE TO GROWTH-FACTORS
THE SMALL GTP-BINDING PROTEIN RHO REGULATES THE ASSEMBLY OF FOCAL ADHESIONS AND ACTIN STRESS FIBERS IN RESPONSE TO GROWTH-FACTORS
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DOI：
10.1016/0092-8674(92)90163-7
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发表时间：
1992-08-07
期刊：
影响因子：
64.5
通讯作者：
HALL, A
中科院分区：
文献类型：
--
作者：
RIDLEY, AJ;HALL, A
肌动蛋白应激纤维是成纤维细胞中主要的细胞骨架结构之一，通过局灶黏附与质膜相连。rho是一种ras相关的gtp结合蛋白，将rho微量注射到血清饥饿的Swiss 3T3细胞中，可迅速刺激应激纤维和局灶黏附形成。重新检测血清也产生了类似的反应，在2分钟内就可以检测到。这种活性是由于溶血磷脂（很可能是溶血磷脂酸）与血清白蛋白结合。包括PDGF在内的其他生长因子最初诱导肌动蛋白重组形成膜褶，随后在5至10分钟后形成应力纤维。当内源性rho功能被阻断时，所有被测试的生长因子对局灶黏附和应力纤维组装的刺激都被抑制，而膜褶皱则不受影响。这些数据表明，rho对于生长因子诱导的黏附和应力纤维的协调组装是至关重要的。
Actin stress fibers are one of the major cytoskeletal structures in fibroblasts and are linked to the plasma membrane at focal adhesions. rho, a ras-related GTP-binding protein, rapidly stimulated stress fiber and focal adhesion formation when microinjected into serum-starved Swiss 3T3 cells. Readdition of serum produced a similar response, detectable within 2 min. This activity was due to a lysophospholipid, most likely lysophosphatidic acid, bound to serum albumin. Other growth factors including PDGF induced actin reorganization initially to form membrane ruff les, and later, after 5 to 10 min, stress fibers. For all growth factors tested the stimulation of focal adhesion and stress fiber assembly was inhibited when endogenous rho function was blocked, whereas membrane ruffling was unaffected. These data imply that rho is essential specifically for the coordinated assembly of focal adhesions and stress fibers induced by growth factors.