Author Correction: A surface endogalactanase in Bacteroides thetaiotaomicron confers keystone status for arabinogalactan degradation.
Author Correction: A surface endogalactanase in Bacteroides thetaiotaomicron confers keystone status for arabinogalactan degradation.
复制标题
作者更正：多形拟杆菌中的表面内切半乳聚糖酶赋予阿拉伯半乳聚糖降解的关键地位。
DOI：
10.1038/s41564-019-0584-5
复制
发表时间：
2019
影响因子：
28.3
通讯作者：
Cartmell A
中科院分区：
文献类型：
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作者：
Cartmell A
在这篇文章最初发表的版本中，我们报告了蛋白质BT3679是α-l-阿拉伯呋喃糖苷酶。最近的研究表明，BT3679没有明显的催化活性，因此仍然是一个未知功能的蛋白质。这个错误是在约克大学的Gideon Davies实验室发现的，他试图获得与适当配体复合的BT3679的晶体结构。他的小组独立于我们的工作产生了BT3679，并表明该蛋白质没有表现出α-l-阿拉伯呋喃糖苷酶活性。一旦注意到这种可能性，我们就探索了BT3679主储备液中的质粒群体。将储备液转化到大肠杆菌中并在五个单菌落中对质粒进行测序，结果显示储备液含有两种质粒：BT 3679和BT 0348。先前显示BT 0348质粒编码GH51 α-l-阿拉伯呋喃糖苷酶（Nat.Microbiol.2004）。3，210-119; 2017）。因此，报告的α-l-阿拉伯呋喃糖苷酶活性包括BT 0348，但不包括BT 3679。戴维斯实验室分析了一个蛋白质样品，证实了这一点，该蛋白质是通过固定化金属亲和色谱法从大肠杆菌中纯化出来的。结果表明，α-l-阿拉伯呋喃糖苷酶探针标记的51 kDa蛋白质与BT 0348质粒的大小相对应，但与47 kDa BT 3679条带不对应，因此对该文章进行了如下修改：（1）从图1a、B所示的降解模型中删除了关于BT 3679的信息。已删除补充图7 b、c的子图，并已从补充图8中删除涉及BT3679的面板;已修改相应的标题以反映此类变化。关于BT3679的信息也已从补充表2中删除。(2)图1c和补充表1中已将BT3679指定为一种没有可检测活性的蛋白质。(3)其他提及BT3679的文本已从该条中删除。
In the version of this Article originally published, we reported the protein BT3679 to be an α-l-arabinofuranosidase. Recent studies have shown that BT3679 has no demonstrable catalytic activity and thus remains a protein of unknown function. This error was detected in the laboratory of Gideon Davies, at the University of York, who was attempting to obtain the crystal structure of BT3679 in complex with appropriate ligands. His group generated BT3679 independently of our work, and showed that the protein displayed no α-l-arabinofuranosidase activity. Once alerted to this possibility, we explored the plasmid population in the master stock of BT3679. Transforming the stock into Escherichia coli and sequencing the plasmid in five single colonies showed that the stock contained two plasmids: BT3679 and BT0348. The BT0348 plasmid was previously shown to encode a GH51 α-l-arabinofuranosidase (Nat. Microbiol. 3, 210–119; 2017). Thus, the reported α-l-arabinofuranosidase activity comprised BT0348, but not BT3679. This was confirmed by the Davies laboratory when they analysed a sample of the protein, purified by immobilized metal affinity chromatography from E. coli containing the contaminated BT3679 plasmid preparation; they showed that an α-l-arabinofuranosidase probe labelled a 51 kDa protein corresponding to the size of the BT0348 plasmid, but not to the 47 kDa BT3679 band.The Article has thus been amended as follows:(1) Information regarding BT3679 has been removed from the degradative model shown in Fig. 1a, b. Supplementary Fig. 7b, c sub-figures have been removed, and panels referring to BT3679 have been removed from Supplementary Fig. 8; the respective captions have been amended to reflect such changes. Information regarding BT3679 has also been removed from Supplementary Table 2.(2) The designation of BT3679 as a protein of no detectable activity has been stated in Fig. 1c and Supplementary Table 1.(3) Other text referring to BT3679 has been deleted from the Article.