Preparation and evaluation of PEO-coated materials for a microchannel hemodialyzer.
Preparation and evaluation of PEO-coated materials for a microchannel hemodialyzer.
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微通道血液透析器用PEO涂层材料的制备与评价
DOI：
10.1002/jbm.b.33082
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
McGuire,Joseph
中科院分区：
文献类型：
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作者：
Heintz,Keely;Schilke,KarlF;Snider,Joshua;Lee,Woo-Kul;Truong,Mitchell;Coblyn,Matthew;Jovanovic,Goran;McGuire,Joseph
与用于血液透析的微型器械相关的表面积与体积比的显著增加导致血液相容性和血流分布问题，这些问题比传统规模下遇到的问题更具管理挑战性。在这项工作中，在用于构建微通道血液透析器供试品的聚二甲基硅氧烷（PDMS）、聚碳酸酯微通道和聚丙烯腈膜材料上产生了具有悬垂聚环氧乙烷（PEO）链的稳定表面改性。通过将PEO-聚丁二烯-PEO（PEO-PB-PEO）三嵌段聚合物辐射接枝到材料表面来制备PEO层。通过测量未涂覆和PEO涂覆的表面与β-半乳糖苷酶接触后的表面结合酶活性来评价蛋白质排斥。PEO涂层聚碳酸酯和PDMS材料上的蛋白质吸附降低至未涂层材料上记录的水平的约20%。无论是三嵌段，也没有观察到照射过程中有任何影响蛋白质与聚丙烯腈膜的相互作用，或其对尿素的渗透性。这种方法有望作为安全、有效的涂层原位应用于血液处理的微流体装置的手段，这将确保良好的血液相容性和血流分布，对传质没有不利影响。© 2013 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，102 B：1014-1020，2014。
The marked increase in surface‐to‐volume ratio associated with microscale devices for hemodialysis leads to problems with hemocompatibility and blood flow distribution that are more challenging to manage than those encountered at the conventional scale. In this work stable surface modifications with pendant polyethylene oxide (PEO) chains were produced on polydimethylsiloxane (PDMS), polycarbonate microchannel, and polyacrylonitrile membrane materials used in construction of microchannel hemodialyzer test articles. PEO layers were prepared by radiolytic grafting of PEO‐polybutadiene‐PEO (PEO‐PB‐PEO) triblock polymers to the material surfaces. Protein repulsion was evaluated by measurement of surface‐bound enzyme activity following contact of uncoated and PEO‐coated surfaces withβ‐galactosidase. Protein adsorption was decreased on PEO‐coated polycarbonate and PDMS materials to about 20% of the level recorded on the uncoated materials. Neither the triblocks nor the irradiation process was observed to have any effect on protein interaction with the polyacrylonitrile membrane, or its permeability to urea. This approach holds promise as a means forin situapplication of safe, efficacious coatings to microfluidic devices for blood processing that will ensure good hemocompatibility and blood flow distribution, with no adverse effects on mass transfer. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 1014–1020, 2014.