Accurate and Real-Time Detection Method for the Exothermic Behavior of Enzymatic Nano-Microregions Using Temperature-Sensitive Amino-AgInS2 Quantum Dots.
Accurate and Real-Time Detection Method for the Exothermic Behavior of Enzymatic Nano-Microregions Using Temperature-Sensitive Amino-AgInS2 Quantum Dots.
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DOI：
10.1002/smtd.202100811
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发表时间：
2021-11
期刊：
影响因子：
12.4
通讯作者：
Hui Zhang;Youshen Wu;Peng Tang;Hongrui Zhu;Zhenhai Gan;Hu-Qin Zhang;Daocheng Wu
中科院分区：
文献类型：
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作者：
Hui Zhang;Youshen Wu;Peng Tang;Hongrui Zhu;Zhenhai Gan;Hu-Qin Zhang;Daocheng Wu
酶在纳米尺度下的热行为对生命现象具有重要意义。酶在纳米尺度上的这种非平衡态实时热行为无法用现有的方法精确检测。本文提出了一种检测这种热行为的新方法。酶-量子点（QD）偶联物可以通过将温度敏感的氨基agins2量子点化学接枝到酶上得到，其中量子点作为纳米温度计，灵敏度为-2.82%°C-1。通过检测酶- qd偶联物的光致发光强度变化，可以得到酶的实时热行为。酶- qd偶联物在催化过程中，在纳米微区与环境温度的温差高达6℃，重现性好（最大误差为4%），而溶液温度几乎没有变化。该方法的温度分辨率为≈0.5℃，酶的检出限为0.02 mg mL-1，在空间上保证了氨基- agins2量子点与酶的定量结合；因此，它可以准确地检测酶的放热行为，并可以推广到其他生物的检测中。该方法灵敏度高，稳定性好，可靠性高，在研究纳米尺度生物热行为及相关生命现象方面具有很大的应用潜力。
The thermal behavior of enzymes in nanoscale is of great significance to life phenomena. This nonequilibrium state real-time thermal behavior of enzymes at nanoscale cannot be accurately detected by existing methods. Herein, a novel method is developed for the detection of this thermal behavior. The enzyme-quantum dot (QD) conjugates can be obtained by chemically grafting temperature-sensitive amino-AgInS2 QDs to the enzyme, where the QDs act as nanothermometers with a sensitivity of -2.82% °C-1 . Detecting the photoluminescence intensity changes of the enzyme-QD conjugates, the real-time thermal behavior of enzymes can be obtained. The enzyme-QD conjugates show a temperature difference as high as 6 °C above ambient temperature in nano-microregions with good reproducibility (maximum error of 4%) during catalysis, while solution temperature hardly changed. This method has a temperature resolution of ≈0.5 °C with a detection limit of 0.02 mg mL-1 of enzyme, and spatially ensured that the amino-AgInS2 QDs are quantitatively bound to the enzyme; thus, it can accurately detect the exothermic behavior of the enzyme and can be extended to other organisms' detection. This method has high sensitivity, good stability, and reliability, indicating its great potential application in investigating the thermal behavior of organisms in nanoscale and related life phenomena.