Effects of treadmill running and rutin on lipolytic signaling pathways and TRPV4 protein expression in the adipose tissue of diet-induced obese mice
Effects of treadmill running and rutin on lipolytic signaling pathways and TRPV4 protein expression in the adipose tissue of diet-induced obese mice
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跑步机跑步和芦丁对饮食诱导肥胖小鼠脂肪组织中脂肪分解信号通路和 TRPV4 蛋白表达的影响。
DOI：
10.1007/s13105-015-0437-5
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发表时间：
2015-12-01
影响因子：
3.4
通讯作者：
Wan, Zhongxiao
中科院分区：
文献类型：
--
作者：
Chen, Neng;Cheng, Jinbo;Wan, Zhongxiao
探讨芦丁和运动对高脂饮食（HFD）诱导的脂肪分解信号、腺苷酸（AMP）激活的蛋白激酶（AMPK）信号、瞬时受体电位阳离子通道亚家族V成员4（TRPV 4）及其相关蛋白表达的影响，以及是否存在贮库特异性效应。C57 BL/6 J小鼠随机分为5组：普通饲料组、高脂饮食组、高脂饮食+芦丁干预组、高脂饮食+跑台运动组、高脂饮食+跑台运动+芦丁干预组。在16周干预结束时，通过蛋白质印迹法测量脂肪组织的脂解标志物、AMPK信号通路、TRPV 4和过氧化物酶体增殖物激活受体γ辅激活因子1 α + β（PGC-1 α + β）。在附睾脂肪组织中，HFD导致丝氨酸660（p-HSL 660）处的激素敏感性脂肪酶、围脂蛋白A、磷酸烯醇丙酮酸羧激酶（PEPCK）、p-AMPK和p-乙酰辅酶A羧化酶（ACC）蛋白表达的磷酸化显著降低。运动干预和运动加芦丁完全恢复p-HSL 660、perilipin A、PEPCK、p-AMPK和p-ACC蛋白表达至正常水平。HFD组和HR组PGC-1 α + β表达降低，运动组和运动加芦丁组PGC-1 α + β表达完全恢复至正常水平。在皮下脂肪组织中，HFD升高TRPV 4，运动，运动加芦丁完全降低TRPV 4至正常水平。HR、HE和HRE组PGC-1 α + β增加。总之，关于芦丁和运动如何影响脂解信号和p-AMPK以及TRPV 4和PGC-1 α + β表达，存在储存库特异性效应。
To explore the effects of rutin and exercise on high-fat diet (HFD)-induced disrupted lipolytic signaling, adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) signaling, transient receptor potential cation channel subfamily V member 4 (TRPV4) and its associated protein expression, and whether depot-specific effects existed. C57BL/6J mice were randomized into five groups: chow group, HFD, HFD plus rutin intervention group (HR), HFD combined with treadmill running group (HE), and HFD combined with treadmill running and rutin intervention group (HRE). At the end of the 16-week intervention, lipolytic markers, AMPK signaling pathways, TRPV4, and peroxisome proliferator-activated receptor gamma coactivator 1 alpha + beta (PGC-1 alpha + beta) from adipose tissue were measured by western blotting. In epididymal adipose tissue, HFD resulted in significant reduction in the phosphorylation of hormone sensitive lipase at serine660 (p-HSL660), perilipin A, phosphoenolpyruvate carboxykinase (PEPCK), p-AMPK, and p-acetyl-CoA carboxylase (ACC) protein expression. Exercise intervention and exercise plus rutin completely restored p-HSL660, perilipin A, PEPCK, p-AMPK, and p-ACC protein expression to normal level. HFD and HR groups have reduced expression of PGC-1 alpha + beta, exercise, and exercise plus rutin completely restored PGC-1 alpha + beta expression to normal level. In subcutaneous adipose tissue, HFD elevated TRPV4, exercise, and exercise plus rutin completely reduced TRPV4 to normal level. HR, HE, and HRE group have increased PGC-1 alpha + beta. In conclusion, depot-specific effects existed in regards to how rutin and exercise affect lipolytic signaling and p-AMPK, as well as TRPV4 and PGC-1 alpha + beta expression.