Antibacterial activity of lime (Citrus aurantifolia) essential oil and limonene against fish pathogenic bacteria isolated from cultured olive flounder (Paralichthys olivaceus)
Antibacterial activity of lime (Citrus aurantifolia) essential oil and limonene against fish pathogenic bacteria isolated from cultured olive flounder (Paralichthys olivaceus)
复制标题
石灰（Citrus aurantifolia）精油和柠檬烯对从养殖橄榄鲽（Paralichthys olivaceus）中分离出的鱼类病原菌的抗菌活性
作者：
H. Pathirana;S. Wimalasena;B. D. De Silva;S. Hossain;G. Heo
摘要 测试了酸橙 (Citrus aurantifolia) 精油 (LEO) 和柠檬烯对从韩国养殖的橄榄鲽、Paralichthys olivaceus (Temminck & Schlegel) 中分离出的 7 种革兰氏阴性和 9 种革兰氏阳性鱼类病原菌的抗菌活性。柠檬烯的浓度 >99%，LEO 由 11 种化合物组成，其中柠檬烯含量为 56.22%。进行纸片扩散测定、最低抑菌浓度（MIC）和最低杀菌浓度（MBC）测试。 LEO 和柠檬烯抑制革兰氏阴性菌和革兰氏阳性菌的生长。 LEO 和柠檬烯 (MBC/MIC=2-8) 对测试菌株均具有杀菌和抑菌作用。在每种鱼类致病菌中，抑菌圈直径 (IZD) 与油浓度成比例增加，并且在 LEO 和柠檬烯浓度为 100% (V/V) 时发现最大效果。抗菌谱图谱表明，除海豚链球菌的三种菌株（S186、S530 和 S131）外，所有细菌菌株均显示出对一种或多种抗生素的耐药性。相对抑菌圈直径的百分比（RIZD%）在所有试剂的浓度较高时表现出较高的值。由于LEO和柠檬烯的抗菌活性对鱼类致病菌相当有效，因此它们可以用作治疗水产养殖中细菌感染的替代品。
Abstract The antibacterial activity of lime (Citrus aurantifolia) essential oil (LEO) and limonene was tested against seven Gram-negative and nine Gram-positive fish pathogenic bacteria isolated from cultured olive flounder, Paralichthys olivaceus (Temminck & Schlegel) in Korea. Limonene was >99% concentrated and LEO consisted of eleven chemical compounds including 56.22% of limonene. Disk diffusion assay, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) tests were done. LEO and limonene inhibited the growth of both Gram-negative and Gram-positive bacteria. LEO and limonene (MBC/MIC= 2-8) were both bactericidal and bacteriostatic for the strains tested. In every fish pathogenic bacteria, the inhibition zone diameter (IZD) increased in proportion to the oil concentration and the maximum effect was found at 100% (V/V) concentrations of LEO and limonene. The antibiogram pattern indicated that all the bacterial strains, excluding three strains of S. iniae (S186, S530, and S131), showed resistance to one or more antibiotics. The percentage of the relative inhibition zone diameter (RIZD %) exhibited high values at higher concentrations of all the agents. Since antibacterial activities of LEO and limonene were considerably effective against fish pathogenic bacteria, they could be used as alternatives to treat bacterial infections in aquaculture.
影响因子：
56.9
作者：
IMLAY, JA;CHIN, SM;LINN, S
通讯作者：
LINN, S