Brain-wide maps of Fos expression during fear learning and recall
Brain-wide maps of Fos expression during fear learning and recall
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DOI：
10.1101/lm.044446.116
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发表时间：
2017-04-01
影响因子：
2
通讯作者：
Gray, Jesse M.
中科院分区：
文献类型：
--
作者：
Cho, Jin-Hyung;Rendall, Sam D.;Gray, Jesse M.
学习过程中的Fos诱导标记了海马体中编码特定物理环境的神经元集合，揭示了记忆痕迹。在大脑皮层和其他区域，学习过程中Fos诱导在多大程度上揭示了特定的感觉表征尚不清楚。在这里，我们生成高质量的全脑Fos mRNA表达的地图，在听觉恐惧条件反射和回忆的设置的家庭笼。这些地图揭示了一种全脑范围的Fos诱导模式，这种模式在恐惧条件反射、仅电击、仅音调和恐惧回忆条件下非常相似，这让人对Fos揭示了特定于大脑的感觉表征的想法产生了怀疑。事实上，新的听觉音调在视觉皮层和听觉皮层中诱导的基因一样多，而熟悉的（非条件）音调在大脑的任何地方都不会明显诱导Fos。Fos表达水平与身体活动无关，这表明它们不是由感觉体验中的行为活动驱动的改变决定的。在丘脑中，Fos在边缘系统比在感觉中继核中更显著地被诱导，这表明Fos可能对情绪状态最敏感。因此，我们的数据表明，Fos表达在简单的联想学习标签合奏激活一般由唤醒，而不是特定的感官线索。
Fos induction during learning labels neuronal ensembles in the hippocampus that encode a specific physical environment, revealing a memory trace. In the cortex and other regions, the extent to which Fos induction during learning reveals specific sensory representations is unknown. Here we generate high-quality brain-wide maps of Fos mRNA expression during auditory fear conditioning and recall in the setting of the home cage. These maps reveal a brain-wide pattern of Fos induction that is remarkably similar among fear conditioning, shock-only, tone-only, and fear recall conditions, casting doubt on the idea that Fos reveals auditory-specific sensory representations. Indeed, novel auditory tones lead to as much gene induction in visual as in auditory cortex, while familiar (nonconditioned) tones do not appreciably induce Fos anywhere in the brain. Fos expression levels do not correlate with physical activity, suggesting that they are not determined by behavioral activity-driven alterations in sensory experience. In the thalamus, Fos is induced more prominently in limbic than in sensory relay nuclei, suggesting that Fos may be most sensitive to emotional state. Thus, our data suggest that Fos expression during simple associative learning labels ensembles activated generally by arousal rather than specifically by a particular sensory cue.