The pig eye as a novel model of glaucoma
The pig eye as a novel model of glaucoma
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DOI：
10.1016/j.exer.2005.03.014
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发表时间：
2005-11-01
影响因子：
3.4
通讯作者：
Vecino, E
中科院分区：
文献类型：
--
作者：
Ruiz-Ederra, J;García, M;Vecino, E
基于慢性眼压升高（IOP），我们验证了猪眼作为青光眼的模型。通过烧灼5只成年猪左眼的3条外膜静脉，提高IOP。右眼作为对照。实验期间用压平眼压计（Tono-Pen）测量眼压。在巩膜外静脉闭塞5个月后，用氟金逆行充填烧灼眼和对照眼的视网膜神经节细胞（RGCs）。RGC损失分析和形态计量学作为存活RGC的特征，使用整个视网膜进行。经三周外膜静脉烧灼治疗后，IOP明显升高，且升高至少持续21周（实验持续时间）。慢性IOP升高后的RGC损失分析显示，RGC死亡在视网膜中周和外周都很明显，主要发生在两个视网膜区域的时间象限。此外，观察到剩余RGCs的平均体细胞面积增加，我们发现中外周和外周视网膜中较大RGCs的损失更大。我们的结论是，猪视网膜上膜静脉烧灼引起的RGC死亡模式与人类青光眼相似。基于本研究，基于猪视网膜与人类视网膜的相似性和可负担性，猪视网膜可能被认为是青光眼相关研究的合适模型。(c) 2005 Elsevier Ltd版权所有。
We validated the pig eye as a model of glaucoma, based on chronic elevation of intraocular pressure (IOP). IOP was elevated by cauterising three episcleral veins in each of the left eyes of five adult pigs. Right eyes were used as controls. Measurement of IOP was performed during the experiment with an applanation tonometer (Tono-Pen). Five months after episcleral vein occlusion, retinal ganglion cells (RGCs) from both cauterised and control eyes were retrogradely backfilled with Fluoro-Gold. Analysis of RGC loss and morphometric as characterization of surviving RGCs was performed using whole-mounted retinas. Elevation of IOP was apparent after three weeks of episcleral vein cauterisation and it remained elevated for at least 21 weeks (duration of the experiments). Analysis of RGC loss after chronic elevation of IOP revealed that RGC death was significant in the mid-peripheral and peripheral retina, mainly in the temporal quadrants of both retinal regions. Moreover the mean soma area of remaining RGCs was observed to increase and we found a greater loss of large RGCs in the mid-peripheral and peripheral retina. We conclude that the pattern of RGC death induced in the pig retina by episcleral vein cauterisation resembles that found in human glaucoma. On the basis of this study, the pig retina may be considered as a suitable model for glaucoma-related studies, based on its similarity with human and on its affordability. (c) 2005 Elsevier Ltd. All rights reserved.