Prrx1 marks stem cells for bone, white adipose tissue and dermis in adult mice
Prrx1 marks stem cells for bone, white adipose tissue and dermis in adult mice
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DOI：
10.1038/s41588-022-01227-4
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发表时间：
2022-12-01
期刊：
影响因子：
30.8
通讯作者：
Li, Baojie
中科院分区：
文献类型：
--
作者：
Liu, Huijuan;Li, Ping;Li, Baojie
特殊的结缔组织，包括骨和脂肪组织，控制着各种生理活动，包括矿物质和能量的动态平衡。然而，干细胞在成年期维持这些组织的身份仍然难以捉摸。通过对新产生的敲入Cre/Creer(T2)细胞系进行遗传谱系追踪和细胞耗竭实验，我们发现在成年小鼠中，罕见的Prrx1表达细胞作为骨、白色脂肪组织和真皮的干细胞，这些组织的动态平衡和修复是不可或缺的。单细胞图谱揭示了Prrx1表达细胞的周期性和多能性，这些细胞的干细胞特性通过移植试验得到了进一步的验证。此外，我们确定了表达Prrx1的干细胞的细胞表面标志，并表明这些干细胞的活性受Wnt信号的调节。这些发现扩大了我们对结缔组织动态平衡/再生的了解，并可能有助于改进基于干细胞的治疗。Prrx1的表达可以作为成年小鼠骨、白色脂肪组织和真皮的干细胞。这些细胞在组织的动态平衡和修复中起关键作用，并受Wnt信号的调节。
Specialized connective tissues, including bone and adipose tissues, control various physiological activities, including mineral and energy homeostasis. However, the identity of stem cells maintaining these tissues throughout adulthood remains elusive. By conducting genetic lineage tracing and cell depletion experiments in newly generated knock-in Cre/CreER(T2) lines, we show here that rare Prrx1-expressing cells act as stem cells for bone, white adipose tissue and dermis in adult mice, which are indispensable for the homeostasis and repair of these tissues. Single-cell profiling reveals the cycling and multipotent nature of Prrx1-expressing cells and the stemness of these cells is further validated by transplantation assays. Moreover, we identify the cell surface markers for Prrx1-expressing stem cells and show that the activities of these stem cells are regulated by Wnt signaling. These findings expand our knowledge of connective tissue homeostasis/regeneration and may help improve stem-cell-based therapies.Prrx1-expressing can act as stem cells for bone, white adipose tissue and dermis in adult mice. These cells have a key role in tissue homeostasis and repair and are regulated by Wnt signaling.