Structure of the conserved domain of ANAC, a member of the NAC family of transcription factors
Structure of the conserved domain of ANAC, a member of the NAC family of transcription factors
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DOI：
10.1038/sj.embor.7400093
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发表时间：
2004-03-01
期刊：
影响因子：
7.7
通讯作者：
Lo Leggio, L
中科院分区：
文献类型：
--
作者：
Ernst, HA;Olsen, AN;Lo Leggio, L
拟南芥ANAC（脱落酸响应性NAC）的DNA结合NAC结构域的结构已通过X射线晶体学确定至1.9埃分辨率（蛋白质数据库代码1UT 4和1UT 7）。这是第一个确定的植物特异性转录调节因子的NAC家族成员的结构。NAC蛋白的特征在于其保守的N-末端NAC结构域，其可以结合DNA和其他蛋白质。NAC蛋白参与发育过程，包括茎顶端分生组织、花器官和侧枝的形成，以及植物激素控制和防御。NAC结构域不具有经典的螺旋-转角螺旋基序;相反，它揭示了一种新的转录因子折叠，由一些螺旋元件包围的扭曲的β-折叠组成。NAC结构域形成的功能性二聚体在结构上得到了鉴定，这将作为在分子水平上理解NAC蛋白功能的结构模板。
The structure of the DNA-binding NAC domain of Arabidopsis ANAC (abscisic-acid-responsive NAC) has been determined by X-ray crystallography to 1.9Angstrom resolution (Protein Data Bank codes 1UT4 and 1UT7). This is the first structure determined for a member of the NAC family of plant-specific transcriptional regulators. NAC proteins are characterized by their conserved N-terminal NAC domains that can bind both DNA and other proteins. NAC proteins are involved in developmental processes, including formation of the shoot apical meristem, floral organs and lateral shoots, as well as in plant hormonal control and defence. The NAC domain does not possess a classical helix-turn helix motif; instead it reveals a new transcription factor fold consisting of a twisted beta-sheet surrounded by a few helical elements. The functional dimer formed by the NAC domain was identified in the structure, which will serve as a structural template for understanding NAC protein function at the molecular level.