Amplification of 'variola virus-specific' sequences in German cowpox virus isolates
Amplification of 'variola virus-specific' sequences in German cowpox virus isolates
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DOI：
10.1046/j.1439-0450.2002.00532.x
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发表时间：
2002-02-01
期刊：
影响因子：
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通讯作者：
Pfeffer, M
中科院分区：
文献类型：
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作者：
Meyer, H;Neubauer, H;Pfeffer, M
1995 年，Knight 等人发表了聚合酶链式反应 (PCR) 方案，描述了天花病原体天花病毒的特异性检测。由于扩增子大小不同，强毒力天花主要菌株可以与弱毒力天花次要菌株区分开来。在这里，我们将此 PCR 方案应用于来自各种正痘病毒分离株的 DNA。正痘病毒种牛痘、猴痘、鼠痘或骆驼痘病毒没有扩增。然而，在所研究的 15 种牛痘病毒株中，有 6 种观察到了扩增。扩增子的大小与天花小毒株描述的大小完全一致，并且核苷酸序列同一性占97%。研究结果讨论了正痘病毒物种的进化，假设天花病毒很可能源自啮齿动物传播的牛痘病毒样祖细胞。
In 1995 a polymerase chain reaction (PCR) protocol describing the specific detection of variola virus, the causative agent of smallpox, was published by Knight and others. Virulent variola major strains could be differentiated from less virulent variola minor strains because of the distinct amplicon sizes. Here, we applied this PCR protocol to DNA from various orthopoxvirus isolates. There was no amplification with the orthopoxvirus species vaccinia, monkeypox, mousepox, or camelpox viruses. However, amplification was observed in six out of 15 cowpox virus strains investigated. The size of the amplicons corresponded exactly with the size described for variola minor strains and the nucleotide sequence identity accounted for 97%. Findings are discussed with respect to the evolution of orthopoxvirus species assuming that variola virus most probably stems from a rodent-transmitted cowpox virus-like progenitor.