Tension applied through the Dam1 complex promotes microtubule elongation providing a direct mechanism for length control in mitosis
Tension applied through the Dam1 complex promotes microtubule elongation providing a direct mechanism for length control in mitosis
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DOI：
10.1038/ncb1609
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发表时间：
2007-07-01
影响因子：
21.3
通讯作者：
Asbury, Charles L.
中科院分区：
文献类型：
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作者：
Franck, Andrew D.;Powers, Andrew F.;Asbury, Charles L.
在分裂细胞中，着丝粒将染色体与生长和缩短的微管纤维的尖端耦合(1,2)，着丝粒-微管界面的张力促进纤维伸长(3-6)。张力依赖性微管纤维伸长被认为对于协调染色体排列和分离至关重要(1,3,7-10)，但这种效应的机制尚不清楚。使用光镊，我们对动粒-微管界面模型施加张力，该模型由酵母 Dam1 复合物 (11-13) 与单个动态微管尖端 (14) 结合组成。较高的张力降低了正在生长的尖端开始缩短的可能性，减缓了缩短，并增加了缩短的尖端恢复生长的可能性。这些效应与许多细胞类型中张力对着丝粒附着的微管纤维的影响相似，表明我们已经重建了有丝分裂中微管长度控制的直接机制。
In dividing cells, kinetochores couple chromosomes to the tips of growing and shortening microtubule fibres(1,2) and tension at the kinetochore-microtubule interface promotes fibre elongation(3-6). Tension-dependent microtubule fibre elongation is thought to be essential for coordinating chromosome alignment and separation(1,3,7-10), but the mechanism underlying this effect is unknown. Using optical tweezers, we applied tension to a model of the kinetochore-microtubule interface composed of the yeast Dam1 complex(11-13) bound to individual dynamic microtubule tips(14). Higher tension decreased the likelihood that growing tips would begin to shorten, slowed shortening, and increased the likelihood that shortening tips would resume growth. These effects are similar to the effects of tension on kinetochore-attached microtubule fibres in many cell types, suggesting that we have reconstituted a direct mechanism for microtubule-length control in mitosis.