Production and characterization of a silk-like hybrid protein, based on the polyalanine region of Samia cynthia ricini silk fibroin and a cell adhesive region derived from fibronectin
Production and characterization of a silk-like hybrid protein, based on the polyalanine region of Samia cynthia ricini silk fibroin and a cell adhesive region derived from fibronectin
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DOI：
10.1016/s0142-9612(03)00570-2
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发表时间：
2004-02-01
期刊：
影响因子：
14
通讯作者：
Kurokawa, M
中科院分区：
文献类型：
--
作者：
Asakura, T;Tanaka, C;Kurokawa, M
蚕的种类繁多，并产生丝素蛋白。蚕丝具有许多适合作为生物材料的固有特性。本文设计并制备了一种新的类丝蛋白杂合蛋白[DGG（A）（12）GGAASTGRGDSPAAS]（5），该杂合蛋白由蓖麻蚕丝素蛋白的多聚丙氨酸区和纤维连接蛋白的Arg-Gly-Asp（RGD）区组成。构建了编码该杂合蛋白的基因，并在大肠杆菌中表达。聚丙氨酸区域的主要构象，即α-螺旋或β-折叠，可以通过分别用不同的酸性溶剂三氟乙酸或甲酸处理而容易地控制。用C-13 CP/MAS NMR监测这种结构变化。与胶原蛋白相比，杂交蛋白具有更高的细胞粘附和生长活性。(C)2003爱思唯尔有限公司。保留所有权利。
There are a variety of silkworms and silk fibroins produced by them. Silks have many inherent suitable properties for biomaterials. In this paper, a novel silk-like hybrid protein, [DGG(A)(12)GGAASTGRGDSPAAS](5), which consists of polyalanine region of silk fibroin from a wild silkworm, Samia cynthia ricini, and cell adhesive region including Arg-Gly-Asp (RGD) sequence, derived from fibronectin, was designed and produced. The genes encoding the hybrid protein were constructed and expressed in Escherichia coli. The main conformation of the polyalanine region, that is, either alpha-helix or beta-sheet, could be easily controlled by treatment with different acidic solvents, trifluoroacetic acid or formic acid, respectively. This structural change was monitored with C-13 CP/MAS NMR. Higher cell adhesive and growth activities of the hybrid protein compared with those of collagen were obtained. (C) 2003 Elsevier Ltd. All rights reserved.