Co-localization and regulation of basic fibroblast growth factor and arginine vasopressin in neuroendocrine cells of the rat and human brain.
Co-localization and regulation of basic fibroblast growth factor and arginine vasopressin in neuroendocrine cells of the rat and human brain.
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DOI：
10.1186/1743-8454-7-13
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发表时间：
2010-08-13
影响因子：
--
通讯作者：
Baird A
中科院分区：
文献类型：
--
作者：
Gonzalez AM;Taylor WM;Johanson CE;King JC;Leadbeater WE;Stopa EG;Baird A
成年大鼠下丘脑-垂体轴和脉络丛富含碱性成纤维细胞生长因子（FGF 2），可能在体液平衡中起作用。为此，我们的特点是在人类和大鼠中枢神经系统中的分布和调制的FGF 2。为了确定精氨酸加压素（AVP）和FGF 2之间的功能联系，慢性脱水的大鼠模型被用来测试的假设，即FGF 2的表达，如AVP的表达，是由扰动流体平衡改变。应用免疫组织化学和共聚焦显微镜观察了正常人脑内FGF 2和AVP神经肽的分布。为了评估慢性脱水的影响，将Sprague-Dawley大鼠禁水3天。AVP神经肽的表达和变化，在脑，垂体和肾脏的神经叶的FGF 2分布进行了评估，免疫组化和蛋白质印迹法（FGF 2亚型）。在人下丘脑，FGF 2和AVP共定位于视上和室旁大细胞神经元和轴突突起的细胞质中。免疫反应性FGF 2与分布在整个神经元细胞质中的小颗粒结构相关。神经垂体FGF 2免疫组化染色见于轴突突起、垂体细胞和Herring小体。在大鼠慢性脱水后，血管、垂体细胞和其他胶质细胞下面的基底膜中的FGF 2染色显著增强。这伴随着细胞外基质的重塑。蛋白质印迹分析显示，脱水增加了下丘脑FGF 2亚型的表达。18、23和24 kDa。在脱水大鼠侧脑室脉络丛中，FGF 2在上皮中的表达增加（Ab 773作为免疫标记物），但减少（Ab 106免疫染色）。脱水改变了含AVP的大细胞神经元和神经垂体以及脉络丛上皮中FGF 2的表达模式。这支持了中枢合成的FGF 2参与调节体液平衡的体内平衡机制，其与AVP偶联。
Adult rat hypothalamo-pituitary axis and choroid plexus are rich in basic fibroblast growth factor (FGF2) which likely has a role in fluid homeostasis. Towards this end, we characterized the distribution and modulation of FGF2 in the human and rat central nervous system. To ascertain a functional link between arginine vasopressin (AVP) and FGF2, a rat model of chronic dehydration was used to test the hypothesis that FGF2 expression, like that of AVP, is altered by perturbed fluid balance. Immunohistochemistry and confocal microscopy were used to examine the distribution of FGF2 and AVP neuropeptides in the normal human brain. In order to assess effects of chronic dehydration, Sprague-Dawley rats were water deprived for 3 days. AVP neuropeptide expression and changes in FGF2 distribution in the brain, neural lobe of the pituitary and kidney were assessed by immunohistochemistry, and western blotting (FGF2 isoforms). In human hypothalamus, FGF2 and AVP were co-localized in the cytoplasm of supraoptic and paraventricular magnocellular neurons and axonal processes. Immunoreactive FGF2 was associated with small granular structures distributed throughout neuronal cytoplasm. Neurohypophysial FGF2 immunostaining was found in axonal processes, pituicytes and Herring bodies. Following chronic dehydration in rats, there was substantially-enhanced FGF2 staining in basement membranes underlying blood vessels, pituicytes and other glia. This accompanied remodeling of extracellular matrix. Western blot data revealed that dehydration increased expression of the hypothalamic FGF2 isoforms of ca. 18, 23 and 24 kDa. In lateral ventricle choroid plexus of dehydrated rats, FGF2 expression was augmented in the epithelium (Ab773 as immunomarker) but reduced interstitially (Ab106 immunostaining). Dehydration altered FGF2 expression patterns in AVP-containing magnocellular neurons and neurohypophysis, as well as in choroid plexus epithelium. This supports the involvement of centrally-synthesized FGF2, putatively coupled to that of AVP, in homeostatic mechanisms that regulate fluid balance.