Extensive synthesis of poly[r(G-C)] using Escherichia coli RNA polymerase.
Extensive synthesis of poly[r(G-C)] using Escherichia coli RNA polymerase.
复制标题
使用大肠杆菌 RNA 聚合酶广泛合成聚[r(G-C)]。
DOI：
10.1016/0003-9861(85)90604-6
复制
发表时间：
1985
影响因子：
3.9
通讯作者：
Chamberlin,MJ
中科院分区：
文献类型：
--
作者：
Hall,K;Cruz,P;Chamberlin,MJ
利用大肠杆菌RNA聚合酶以poly[d（I-C）]·poly[d（I-C）]为模板，发现了大量合成poly[r（G-C）]的条件。广泛的合成，其中许多拷贝的模板由酶产生，持续24小时。重复添加模板是合成继续所必需的，因为聚[r（G-C）]似乎与模板结合，抑制聚合酶的转录。使用0.3 mg蛋白质制备了400 ODU或20 mg聚[r（G-C）]。
Conditions have been found that allow for extensive synthesis of poly[r(G-C)] usingEscherichia coliRNA polymerase with a poly[d(I-C)]·poly[d(I-C)] template. The extensive synthesis, in which many copies of the template were produced by the enzyme, continued for 24 h. Repeated addition of the template was necessary for synthesis to continue, as the poly[r(G-C)] appeared to be binding to the template, inhibiting transcription by the polymerase. Four hundred ODU, or 20 mg of the poly[r(G-C)] has been prepared using 0.3 mg of protein.