A replication-selective adenoviral vector for head and neck cancers
A replication-selective adenoviral vector for head and neck cancers
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DOI：
10.1001/archotol.131.7.630
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发表时间：
2005-07-01
影响因子：
--
通讯作者：
Nibu, K
中科院分区：
文献类型：
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作者：
Tanaka, H;Shirakawa, T;Nibu, K
目的：检测基于环氧合酶2(COX-2)启动子的条件复制选择性腺病毒载体对头颈部鳞状细胞癌细胞的溶瘤活性。设计：体外研究。研究对象：无干预：构建了由COX-2启动子调控病毒复制所需E1a表达的条件复制选择腺病毒载体Ad-COX2-E1a。结果：KB、H891、T891、T892和1871的COX-2信使RNA表达比分别为1.5、60.0、1.0、14.6和1.3。体外实验显示COX-2强表达的癌细胞株生长受到明显抑制。结论：本研究证实了COX-2启动子为基础的条件复制选择性腺病毒对COX-2表达的头颈部鳞状细胞癌进行溶瘤治疗的可能性。
Objective: To test the oncolytic activity of cyclooxygenase 2 (COX-2) promoter-based conditional replication-selective adenovirus vector for squamous cell carcinoma cells of the head and neck.Design: In vitro study.Subjects: None.Interventions: A conditional replication-selective adenovirus vector in which the expression of E1a, required for viral replication, is controlled by the COX-2 promoter, Ad-COX2-E1a, was generated. Its oncolytic activity according to the levels of COX-2 and of Coxsackie and adenovirus receptor expression was tested in a series of human head and neck squamous cell carcinoma cell lines.Results: The respective COX-2 messenger RNA expression ratios of KB, H891, T891, T892, and L871 were 1.5, 60.0, 1.0, 14.6, and 1.3. The corresponding Coxsackie and adenovirus receptor messenger RNA expression ratios were 1, 1, 5, 3, and 1. In vitro assays showed significant growth suppression of cancer cell lines with strong expressions of COX-2.Conclusion: This study demonstrated the possibility of oncolytic therapy using the COX-2 promoter-based conditional replication-selective adenovirus for head and neck squamous cell carcinoma expressing COX-2.