Detection of a pheromone-binding protein in the aquatic fungus Achlya ambisexualis.
Detection of a pheromone-binding protein in the aquatic fungus Achlya ambisexualis.
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水生真菌 Achlya ambimaleis 中信息素结合蛋白的检测。
DOI：
10.1016/0014-4827(84)90623-2
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发表时间：
1984
影响因子：
3.7
通讯作者：
McMorris,TC
中科院分区：
文献类型：
--
作者：
Riehl,RM;Toft,DO;Meyer,MD;Carlson,GL;McMorris,TC
Achlya属真核真菌的有性生殖受两种类固醇信息素控制。雄激素是由雌性细胞组成性产生的诱导雄性性分化和发育的信息素。合成了一种具有生物活性的氚标记的蒽二醇衍生物[1，2 - 3H] 7-脱氧-7-二氢-蒽二醇（[3H] 7dA）。放射性配体结合研究表明，在雄性细胞的胞质溶胶中存在一种特异性结合蛋白，可能代表雄激素二醇的内源性受体。与该大分子结合的特征在于表观平衡解离常数和最大结合容量约为1.5g。7 × 10 - 10M和1 100 - 2 000 fmol/mg蛋白质。蔗糖梯度沉降分析表明，结合蛋白分布在8S区域在低离子强度和琥珀酸钠稳定的条件下。在高离子强度条件下，在存在或不存在10 mM Na2SO4的情况下，结合位点分布在梯度的3.6S区域。在其他类固醇和类固醇激素的存在下的放射性配体结合的分析显示，结合是特异性的蒽二酚及其类似物。
Sexual reproduction among the eukaryotic fungi of the genus,Achlya, is controlled by two steroid pheromones. Antheridiol is the pheromone constitutively produced by female cells that induces male sexual differentiation and development. A biologically active tritium-labeled derivative of antheridiol, [1,2-3H]7-deoxy-7-dihydro-antheridiol ([3H]7dA), has been synthesized. Radioligand-binding studies have revealed the presence of a specific binding protein in the cytosol of male cells that may represent the endogenous receptor for antheridiol. Binding to this macromolecule was characterized by an apparent equilibrium dissociation constant and maximum binding capacity of approx. 7 × 10−10M and 1 100–2 000 fmoles/mg protein, respectively. Sedimentation analysis in sucrose gradients revealed that the binding protein distributes in the 8S region under low ionic strength and sodium molybdate-stabilized conditions. Under conditions of high ionic strength, in the presence or absence of 10 mM sodium molybdate, the binding site distributes in the 3.6S region of the gradient. Analysis of radioligand binding in the presence of other steroids and steroid hormones revealed that the binding is specific for antheridiol and its analog.