Calbindin-D28k is a more reliable marker of human Purkinje cells than standard Nissl stains: a stereological experiment.
Calbindin-D28k is a more reliable marker of human Purkinje cells than standard Nissl stains: a stereological experiment.
复制标题
Calbindin-D28k 是比标准尼氏染色更可靠的人类浦肯野细胞标记：体视学实验。
DOI：
10.1016/j.jneumeth.2007.09.009
复制
发表时间：
2008
影响因子：
3
通讯作者：
Blatt,GeneJ
中科院分区：
文献类型：
--
作者：
Whitney,ElizabethR;Kemper,ThomasL;Rosene,DouglasL;Bauman,MargaretL;Blatt,GeneJ
在一项人类浦肯野细胞（PC）数量的研究中，在10个检查的大脑中，有2个发现尼氏染色观察到的PC数量与钙结合蛋白-D28 k（CB）免疫阳性的PC数量之间存在显著的不匹配。在剩下的8个大脑中，没有观察到这种不匹配。此外，在这8个脑中，用尼氏染色复染的CB免疫染色切片的分析显示，超过99%的尼氏染色PC也是CB免疫阳性的。相比之下，在两个不一致的大脑，只有10-20%的CB免疫阳性PC也确定与尼氏染色。虽然这一发现是出乎意料的，但对文献的历史调查显示，Spielmeyer [SpielmeyerW。神经系统组织学朱利叶斯·施普林格：柏林; 1922年。p.56 -79]描述了具有缺乏预期尼氏染色强度的PC的人类病例，这是研究死后人脑时的重要历史发现和关键问题。尼氏染色失败的原因尚不完全清楚，但它可能是由于死前的情况，因为它不是由死后延迟或处理变量。无论确切的原因，这些观察结果表明，尼氏染色可能不是一个可靠的标记PC和CB是一个很好的替代标记。
In a study of human Purkinje cell (PC) number, a striking mismatch between the number of PCs observed with the Nissl stain and the number of PCs immunopositive for calbindin-D28k (CB) was identified in 2 of the 10 brains examined. In the remaining eight brains this mismatch was not observed. Further, in these eight brains, analysis of CB immunostained sections counterstained with the Nissl stain revealed that more than 99% Nissl stained PCs were also immunopositive for CB. In contrast, in the two discordant brains, only 10–20% of CB immunopositive PCs were also identified with the Nissl stain. Although this finding was unexpected, a historical survey of the literature revealed that Spielmeyer [Spielmeyer W. Histopathologie des nervensystems. Julius Springer: Berlin; 1922. p. 56–79] described human cases with PCs that lacked the expected Nissl staining intensity, an important historical finding and critical issue when studying postmortem human brains. The reason for this failure in Nissl staining is not entirely clear, but it may result from premortem circumstances since it is not accounted for by postmortem delay or processing variables. Regardless of the exact cause, these observations suggest that Nissl staining may not be a reliable marker for PCs and that CB is an excellent alternative marker.