Targeting T1 and T2 dual modality enhanced magnetic resonance imaging of tumor vascular endothelial cells based on peptides-conjugated manganese ferrite nanomicelles.
Targeting T1 and T2 dual modality enhanced magnetic resonance imaging of tumor vascular endothelial cells based on peptides-conjugated manganese ferrite nanomicelles.
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基于肽缀合锰铁氧体纳米胶束的肿瘤血管内皮细胞靶向T-1和T-2双模态增强磁共振成像
DOI：
10.2147/ijn.s104686
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发表时间：
2016
影响因子：
8
通讯作者：
Zou L
中科院分区：
文献类型：
--
作者：
Gong M;Yang H;Zhang S;Yang Y;Zhang D;Li Z;Zou L
肿瘤血管生成在肿瘤的发生、发展、转移和预后中起着重要作用。利用MR分子探针对肿瘤血管内皮细胞（TVECs）进行靶向T1/T2双模态磁共振（MR）成像，可以大大提高诊断的敏感性和特异性，有助于在临床前阶段对肿瘤进行早期诊断。在这项研究中，一个新的T1和T2双模纳米探针成功地制造。制备的纳米探针由肽CL 1555、聚（ε-己内酯）-嵌段-聚（乙二醇）两亲性共聚物壳层和数十个锰铁氧体（MnFe 2 O 4）纳米颗粒核组成。结果表明，所制备的疏水性MnFe 2 O 4纳米粒子为均匀的球形，粒径分布窄。由于自组装的纳米胶束结构，所制备的探针具有281.7 mM-1 s-1的高弛豫率，远高于MnFe 2 O 4纳米颗粒的弛豫率（67.5 mM s-1）。在与靶向CD 105肽CL 1555接枝后，纳米胶束可以特异性地与TVEC联合收割机结合，并使标记的TVEC在T2加权MR成像中变暗。随着传代时间的延长，MnFe 2 O 4中的Mn 2+离子逐渐释放，体积逐渐减小，使第2代和第3代标记的TVECs在T1 WI上信号增强。我们的研究结果表明，CL-聚（乙二醇）-MnFe 2 O 4可以共轭TVECs和诱导明暗对比的MR成像，并作为一种新的分子探针T1和T2增强MR成像肿瘤血管生成。
Tumor angiogenesis plays very important roles for tumorigenesis, tumor development, metastasis, and prognosis. Targeting T1/T2 dual modality magnetic resonance (MR) imaging of the tumor vascular endothelial cells (TVECs) with MR molecular probes can greatly improve diagnostic sensitivity and specificity, as well as helping to make an early diagnosis of tumor at the preclinical stage. In this study, a new T1 and T2 dual modality nanoprobe was successfully fabricated. The prepared nanoprobe comprise peptides CL 1555, poly(ε-caprolactone)-block-poly(ethylene glycol) amphiphilic copolymer shell, and dozens of manganese ferrite (MnFe2O4) nanoparticle core. The results showed that the hydrophobic MnFe2O4 nanoparticles were of uniform spheroidal appearance and narrow size distribution. Due to the self-assembled nanomicelles structure, the prepared probes were of high relaxivity of 281.7 mM−1 s−1, which was much higher than that of MnFe2O4 nanoparticles (67.5 mM s−1). After being grafted with the targeted CD105 peptide CL 1555, the nanomicelles can combine TVECs specifically and make the labeled TVECs dark in T2-weighted MR imaging. With the passage on, the Mn2+ ions were released from MnFe2O4 and the size decreased gradually, making the signal intensity of the second and third passage of labeled TVECs increased in T1-weighted MR imaging. Our results demonstrate that CL-poly(ethylene glycol)-MnFe2O4 can conjugate TVECs and induce dark and bright contrast in MR imaging, and act as a novel molecular probe for T1- and T2-enhanced MR imaging of tumor angiogenesis.