Mechanisms underlying microglial colonization of developing neural retina in zebrafish
Mechanisms underlying microglial colonization of developing neural retina in zebrafish
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DOI：
10.1101/2021.05.20.444912
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发表时间：
2021-05
期刊：
影响因子：
7.7
通讯作者：
N. Ranawat;I. Masai
中科院分区：
文献类型：
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作者：
N. Ranawat;I. Masai
小胶质细胞是脑内的巨噬细胞，在脑内起着第一道防线的作用。胚胎小胶质细胞前体起源于外周中胚层，并在发育过程中迁移到脑中。然而，它们在大脑中定居的机制还不完全清楚。视网膜是最早容纳小胶质细胞的大脑区域之一。在斑马鱼中，胚胎小胶质细胞前体使用眼内玻璃体血管作为经由脉络膜裂迁移到视杯的途径。一旦视网膜祖细胞退出细胞周期，与玻璃体血管相关的小胶质细胞前体开始优先通过神经源性区域浸润视网膜，表明视网膜组织的定植取决于神经源性状态。沿着血管和视网膜神经发生，IL 34也参与视网膜的小胶质细胞前体定殖。总之，CSF受体信号传导、血管和神经元分化作为线索发挥作用，以创建小胶质细胞迁移到发育中的视网膜的必要路径。
Microglia are brain-resident macrophages that function as the first line of defense in brain. Embryonic microglial precursors originate in peripheral mesoderm and migrate into the brain during development. However, the mechanism by which they colonize the brain is incompletely understood. The retina is one of the first brain regions to accommodate microglia. In zebrafish, embryonic microglial precursors use intraocular hyaloid blood vessels as a pathway to migrate into the optic cup via the choroid fissure. Once retinal progenitor cells exit the cell cycle, microglial precursors associated with hyaloid blood vessels start to infiltrate the retina preferentially through neurogenic regions, suggesting that colonization of retinal tissue depends upon the neurogenic state. Along with blood vessels and retinal neurogenesis, IL34 also participates in microglial precursor colonization of the retina. Altogether, CSF receptor signaling, blood vessels, and neuronal differentiation function as cues to create an essential path for microglial migration into developing retina.