IR-FEL-induced green fluorescence protein (GFP) gene transfer into plant cell
IR-FEL-induced green fluorescence protein (GFP) gene transfer into plant cell
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DOI：
10.1016/s0168-9002(02)00384-4
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发表时间：
2002-05-01
影响因子：
1.4
通讯作者：
Tamiya, E
中科院分区：
文献类型：
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作者：
Awazu, K;Kinpara, T;Tamiya, E
自由电子激光器（FEL）具有波长可调、脉冲短、峰值功率高等特点，在生物技术领域具有广泛的应用前景。利用红外- fel激光照射将绿色荧光蛋白（GFP）基因成功导入烟草BY2细胞。含有BY2细胞和质粒的溶液辐照面积约为0.1 mm(2)。在5.75和6.1 μ m波长的FEL照射下，分别针对脂质的酯键和蛋白质的酰胺I键的吸收，被证明会导致荧光染料进入细胞。另一方面，GFP荧光仅在5.75 mum照射后瞬间表达。最大传递效率约为0.5%。(C) 2002 Elsevier Science B.V.版权所有
A Free Electron Laser (FEL) holds potential for various biotechnological applications due to its characteristics such as flexible wavelength tunability, short pulse and high peak power. We could successfully introduce the Green Fluorescent Protein (GFP) gene into tobacco BY2 cells by IR-FEL laser irradiation. The irradiated area of the solution containing BY2 cells and plasmid was about 0.1 mm(2). FEL irradiation at a wavelength of 5.75 and 6.1 mum, targeting absorption by the ester bond of the lipid and the amide I bond of the protein, respectively, was shown to cause the introduction of the fluorescent dye into the cell. On the other hand, transient expression of the GFP fluorescence was only observed after irradiation at 5.75 mum. The maximum transfer efficiency was about 0.5%. (C) 2002 Elsevier Science B.V. All rights reserved.