A radioimmunoassay for measurement of serum thymidine.
A radioimmunoassay for measurement of serum thymidine.
复制标题
用于测量血清胸苷的放射免疫测定法。
DOI：
10.1016/0003-2697(73)90137-1
复制
发表时间：
1973
影响因子：
2.9
通讯作者：
B. Stollar
中科院分区：
文献类型：
--
作者：
W. Hughes;M. Christine;B. Stollar
基于胸苷与类似物（~（125）IUdR）竞争抗胸苷抗体上的位点，建立了一种简便、灵敏的血清胸苷放射免疫分析法。在硫酸铵沉淀物中测量抗体结合的125 IUdR。除了浓度高出200倍外，作为正常身体代谢物的结构相关化合物均未显示出胸苷取代标记的能力。在小鼠血清中测得的胸苷浓度略高于10− 6 m。在静脉给药后，跟踪胸苷负荷从血流中消失的速率。该技术的敏感性和特异性进行了讨论，并在放疗和化疗依赖于改变DNA合成或细胞死亡的应用程序已被建议。
A simple and sensitive radioimmunoassay for the measurement of serum thymidine has been developed based on the competition between thymidine and an analog (125IUdR) for sites on anti-thymidine antibody. Antibody-bound125IUdR was measured in ammonium sulfate precipitates. None of the structurally related compounds that are normal body metabolites showed thymidine's ability to displace the label except at 200-fold higher concentration. A thymidine concentration of slightly greater than 10−6m has been measured in mouse blood serum. The rate of disappcarance of a thymidine load from the bloodstream has been followed after intravenous administration. The sensitivity and specificity of the technique have been discussed, and applications in radiotherapy and chemotherapy depending on altered DNA synthesis or cell death have been suggested.