Identification of candidate biomarkers for hepatocellular carcinoma through pre-cancerous expression analysis in an HBx Transgenic mouse
Identification of candidate biomarkers for hepatocellular carcinoma through pre-cancerous expression analysis in an HBx Transgenic mouse
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通过 HBx 转基因小鼠癌前表达分析鉴定肝细胞癌候选生物标志物
DOI：
10.4161/cbt.6.10.4683
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发表时间：
2007-10-01
影响因子：
3.6
通讯作者：
Yang, Xiao
中科院分区：
文献类型：
--
作者：
Sun, Qiang;Zhang, Yingai;Yang, Xiao
本研究的目的是通过HBx转基因小鼠模型中癌前基因表达分析来鉴定用于检测肝细胞癌（HCC）的候选生物标志物。使用DNA寡核苷酸微阵列监测6个月、12个月和18个月大的HBx转基因小鼠和同窝对照小鼠的肝脏和肿瘤组织的基因表达谱。数据分析揭示了HBx转基因小鼠肿瘤组织中684个基因的变化。基于它们的癌前表达谱，鉴定了两个单独的基因组，分别对应于HCC特异性和非特异性组。基因本体分析确定了47个基因编码的分泌或跨膜蛋白中的155个上调基因的肝癌特异性组。其中，与AFP相比，编码TFF 3、IGF 2、LPL和SPP 1的四个基因被发现是用于检测HCC的有希望的生物标志物候选者。这项工作描述了一种新的方法来确定新的生物标志物候选人肝癌诊断。
The aim of this study is to identify candidate biomarkers for the detection of hepatocellular carcinoma (HCC) through pre-cancerous gene expression analysis in an HBx transgenic mouse model. The gene expression profiles of liver and tumor tissues from 6-, 12- and 18-month-old HBx transgenic and littermate control mice were monitored using DNA oligo microarrays. Data analysis revealed changes in 684 genes in the tumor tissues from HBx transgenic mice. Based on their pre-cancerous expression profiles, two separate gene groups, corresponding to HCC specific and non-specific groups respectively, were identified. Gene ontology analysis identified 47 genes encoding secretory or transmembrane proteins among 155 upregulated genes in the HCC-specific group. Among these, four genes encoding TFF3, IGF2, LPL and SPP1 were found to be promising biomarker candidates for the detection of HCC as compared with AFP. This work describes a new way to identify novel biomarker candidates for HCC diagnosis.