B2 adrenergic receptors and morphological changes of the enteric nervous system in colorectal adenocarcinoma.
B2 adrenergic receptors and morphological changes of the enteric nervous system in colorectal adenocarcinoma.
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DOI：
10.3748/wjg.v23.i7.1250
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发表时间：
2017-02-21
影响因子：
4.3
通讯作者：
Vere CC
中科院分区：
文献类型：
--
作者：
Ciurea RN;Rogoveanu I;Pirici D;Târtea GC;Streba CT;Florescu C;Cătălin B;Puiu I;Târtea EA;Vere CC
目的研究原发性结直肠癌肠神经系统的形态学及β 2肾上腺素能受体（B2 A）的表达。在这项研究中，我们纳入了48例原发性结直肠癌患者和9例结肠良性病变切除后的对照组织。我们确定了临床病理特征，并评估了免疫组织化学的表达模式，以及肠神经系统（ENS）的形态学变化。为了评估统计学差异，我们使用学生t检验比较两组的平均值，使用单因素方差分析和Bonferroni事后分析比较两组以上的平均值。使用Pearson相关系数评估相关性。B2 A受体表达与肿瘤分级、肿瘤大小、浸润深度、淋巴结转移有关（P < 0.05），与性别、部位、大体形态无关（P > 0.05）。我们观察到，一方面，随着肿瘤分级的增加，奥尔巴赫丛和迈斯纳丛的相对面积减少，另一方面，随着肿瘤分级的增加，迈斯纳丛或奥尔巴赫丛以外的其他神经元的相对面积增加。对于G1肿瘤，我们发现上皮B2 A面积与奥尔巴赫神经丛面积呈负相关[r（14）=-0.531，P < 0.05]，而对于G2肿瘤，上皮B2 A面积与奥尔巴赫神经丛面积呈间接变化[r（14）=-0.453，P < 0.05]和迈斯纳面积[r（14）=-0.825，P P < 0.01]。对于G3期肿瘤，奥尔巴赫神经丛和迈斯纳神经丛的负相关性均增加[r（14）=-0.587，P < 0.05]和[r（14）=-0.934，P < 0.05]。B2 A受体在结直肠癌的发生中起重要作用，并可作为预后因素。此外，ENS在结直肠癌中的研究可能导致靶向分子治疗。
To study the morphology of the enteric nervous system and the expression of beta-2 adrenergic (B2A) receptors in primary colorectal cancer. In this study, we included forty-eight patients with primary colorectal cancer and nine patients for control tissue from the excision of a colonic segment for benign conditions. We determined the clinicopathological features and evaluated the immunohistochemical expression pattern of B2A receptors as well as the morphological changes of the enteric nervous system (ENS). In order to assess statistical differences, we used the student t-test for comparing the means of two groups and one-way analysis of variance with Bonferroni’s post hoc analysis for comparing the means of more than two groups. Correlations were assessed using the Pearson’s correlation coefficient. B2A receptors were significantly associated with tumor grading, tumor size, tumor invasion, lymph node metastasis (P < 0.05), while there were no statistically significant associations with gender, CRC location and gross appearance (P > 0.05). We observed, on one hand, a decrease of the relative area for both Auerbach and Meissner plexuses with the increase of the tumor grading, and on the other hand, an increase of the relative area of other nervous elements not in the Meissner plexus or in the Auerbach plexus with the tumor grading. For G1 tumors we found that epithelial B2A area showed an inverse correlation with the Auerbach plexus areas [r(14) = -0.531, P < 0.05], while for G2 tumors, epithelial B2A areas showed an indirect variation with both the Auerbach plexus areas [r(14) = -0.453, P < 0.05] and the Meissner areas [r(14) = -0.825, P < 0.01]. For G3 tumors, the inverse dependence increased for both Auerbach [r(14) = -0.587, P < 0.05] and Meissner [r(14) = -0.934, P < 0.05] plexuses. B2A receptors play an important role in colorectal carcinogenesis and can be utilized as prognostic factors. Furthermore, study of the ENS in colorectal cancer may lead to targeted molecular therapies.