NON-ELECTROSTATIC CONTRIBUTION TO THE TITRATION OF THE ORDERED-FLUID PHASE-TRANSITION OF PHOSPHATIDYLGLYCEROL BILAYERS
NON-ELECTROSTATIC CONTRIBUTION TO THE TITRATION OF THE ORDERED-FLUID PHASE-TRANSITION OF PHOSPHATIDYLGLYCEROL BILAYERS
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DOI：
10.1016/0014-5793(80)80313-9
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发表时间：
1980-01-01
期刊：
影响因子：
3.5
通讯作者：
MARSH, D
中科院分区：
文献类型：
--
作者：
CEVC, G;WATTS, A;MARSH, D
磷脂双层的有序流体相变之所以引起人们的兴趣，不仅是因为它提供了生物膜中流体双层区域性质的信息，还因为它可能提供了一种触发膜中有序脂质和流体脂质的横向相分离的方法[1,2]。研究表明[3-61]带负电荷的脂质双分子层的相变在滴定脂质极性头的磷酸基团时表现出强烈的温度变化。这提供了一种通过改变ph值在恒温下触发相变的方法。质子化脂质构成的双分子层的自由能G*= H*-X。7*，脱质子为G*-GPr时的变化，这一变化导致相变温度从q= LUYF/LU~转变为Tt，由下式给出：
The ordered-fluid phase transition of phospholipid bilayers is of interest not only because it yields information on the nature of the fluid bilayer regions in biomembranes, but also because it may provide a means for triggering lateral phase separation between the ordered and fluid lipids in membranes [1, 2]. It has been shown [3-61 that the phase transition of negatively charged lipid bilayers shows strong temperature shifts on titrating the phosphate group of the lipid polar head. This provides a way of triggering the phase transition at constant temperature by varying the pH. The free energy of the bilayer made of protonated lipids, G*= H*-X. 7*, changes upon deprotonation to G*-GPr and this change results in a shift of the phase transition temperature from q= LUYF/LU~ to Tt, given by: