The chromatin scaffold protein SAFB1 localizes SUMO-1 to the promoters of ribosomal protein genes to facilitate transcription initiation and splicing.
The chromatin scaffold protein SAFB1 localizes SUMO-1 to the promoters of ribosomal protein genes to facilitate transcription initiation and splicing.
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DOI：
10.1093/nar/gkv246
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发表时间：
2015-04-20
影响因子：
14.9
通讯作者：
Parvin JD
中科院分区：
文献类型：
--
作者：
Liu HW;Banerjee T;Guan X;Freitas MA;Parvin JD
基因表达的早期步骤是启动子识别、启动子激活、RNA合成和RNA加工的复合过程，众所周知，SUMOylation是一种翻译后修饰，参与转录调控。我们之前发现SUMO-1在人类细胞间期的一些最活跃的内务基因的近端启动子区域标记染色质，但SUMO-1在染色质上的靶点仍不清楚。在本研究中，我们发现SUMO-1通过修饰支架相关因子B （Scaffold Associated Factor B， SAFB）蛋白来标记核糖体蛋白基因的启动子，并且SUMO-1修饰的SAFB刺激了RNA聚合酶与启动子的结合和pre-mRNA剪接。SAFB的缺失减少了RNA聚合酶II与启动子的结合和mRNA的核加工，但mRNA的稳定性不受影响。这项研究揭示了SUMO-1和SAFB在启动子结合、转录起始和RNA加工的刺激偶联中的意想不到的作用。
Early steps of gene expression are a composite of promoter recognition, promoter activation, RNA synthesis and RNA processing, and it is known that SUMOylation, a post-translational modification, is involved in transcription regulation. We previously found that SUMO-1 marks chromatin at the proximal promoter regions of some of the most active housekeeping genes during interphase in human cells, but the SUMOylated targets on the chromatin remained unclear. In this study, we found that SUMO-1 marks the promoters of ribosomal protein genes via modification of the Scaffold Associated Factor B (SAFB) protein, and the SUMOylated SAFB stimulated both the binding of RNA polymerase to promoters and pre-mRNA splicing. Depletion of SAFB decreased RNA polymerase II binding to promoters and nuclear processing of the mRNA, though mRNA stability was not affected. This study reveals an unexpected role of SUMO-1 and SAFB in the stimulatory coupling of promoter binding, transcription initiation and RNA processing.
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