A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus
A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus
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DOI：
10.1099/jmm.0.021956-0
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发表时间：
2010-10-01
影响因子：
3
通讯作者：
Neela, Vasanthakumari
中科院分区：
文献类型：
--
作者：
Ghaznavi-Rad, Ehsanollah;Shamsudin, Mariana Nor;Neela, Vasanthakumari
建立了多重PCR检测耐甲氧西林金黄色葡萄球菌（MRSA）葡萄球菌盒染色体mec （SCCmec）主要型和亚型的方法。该方法采用新型9价多重FOR加2对引物，对金黄色葡萄球菌进行了甲氧西林耐药性鉴定和检测。所有来自马来西亚的389株临床MRSA分离株和来自Harmony收集的18株欧洲MRSA分离株均具有不同的SCCmec类型，我们通过PCR检测正确地鉴定了SCCmec III型和V型是迄今为止医院和社区获得的马来西亚MRSA分离株中最常见的类型，并且明显出现了带有IVh型的MRSA。
A multiplex PCR assay was developed for the identification of major types and subtypes of staphylococcal cassette chromosome mec (SCCmec) in meticillin-resistant Staphylococcus aureus (MRSA) strains. The method uses a novel 9 valent multiplex FOR plus two primer pairs for S. aureus identification and detection of meticillin resistance. All 389 clinical MRSA isolates from Malaysia and 18 European isolates from the Harmony collection harbouring different SCCmec types that we tested were correctly characterized by our PCR assay SCCmec type III and V were by far the most common types among both hospital- and community-acquired Malaysian MRSA isolates, with an apparent emergence of MRSA harbouring the IVh type.