Isolation and characterization of pmk-(1-3): Three p38 homologs in Caenorhabditis elegans
Isolation and characterization of pmk-(1-3): Three p38 homologs in Caenorhabditis elegans
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DOI：
10.1006/mcbr.2001.0300
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发表时间：
2001-11-01
期刊：
影响因子：
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通讯作者：
Cobb, Melanie
中科院分区：
文献类型：
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作者：
Berman, Kevin;McKay, Jim;Cobb, Melanie
p38 是丝裂原激活蛋白激酶 (MAPK) 超家族的成员，可响应多种细胞应激和配体而被激活。由于线虫的基因组已被测序，我们试图鉴定和表征哺乳动物 p38 的线虫同源物。通过序列分析和 RT-PCR，我们分离了编码三种激酶 PMK-1、PMK-2 和 PMK-3 的 cDNA，我们将其称为 p38 图谱激酶，因为它们与 p38 具有高度的序列同一性。这三个基因在 IV 号染色体上相邻，并包含一个操纵子。通过使用 GFP 报告基因，我们发现 pmks 的启动子在整个肠道中都是活跃的。 MAPK/ERK 激酶 6 (MEK6) 的活性形式在体外磷酸化并激活重组 PMK-1 和 PMK-2。 PMK-1 和 PMK-2 磷酸化激活转录因子 2 (ATF-2)，表明其活性与哺乳动物 p38 相似。当转染到哺乳动物细胞中时，这些激酶（如 p38）会受到渗透压的刺激。
p38, a member of the mitogen-activated protein kinase (MAPK) superfamily, is activated in response to a variety of cellular stresses and ligands. Since the genome of the nematode C. elegans has been sequenced, we sought to identify and characterize the nematode homolog of mammalian p38. By sequence analysis and RT-PCR, we isolated cDNAs encoding three kinases, PMK-1, PMK-2, and PMK-3, which we call p38 map kinases due to their high sequence identity with p38. The three genes are contiguous on chromosome IV and comprise an operon. By use of a GFP reporter, we found that the promoter of the pmks is active throughout the intestine. An active form of MAPK/ERK kinase 6 (MEK6) phosphorylated and activated recombinant PMK-1 and PMK-2 in vitro. PMK-1 and PMK-2 phosphorylated activating transcription factor-2 (ATF-2), indicating an activity similar to mammalian p38. When transfected into mammalian cells, these kinases, like p38, are stimulated by osmotic stresses.