MAST-CELL POPULATION-DENSITY, BLOOD-VESSEL DENSITY AND HISTAMINE CONTENT IN NORMAL HUMAN-SKIN
MAST-CELL POPULATION-DENSITY, BLOOD-VESSEL DENSITY AND HISTAMINE CONTENT IN NORMAL HUMAN-SKIN
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DOI：
10.1111/j.1365-2133.1979.tb08065.x
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发表时间：
1979-01-01
影响因子：
10.3
通讯作者：
GREAVES, MW
中科院分区：
文献类型：
--
作者：
EADY, RAJ;COWEN, T;GREAVES, MW
测定了几名健康男性手臂2个部位正常皮肤的肥大细胞群密度，并与同一部位的血管密度和组胺浓度进行了比较。1 ~ 1.5 .mu进行肥大细胞计数和血管计数。M厚塑料切片，通过光镜和组织组胺浓度通过自动荧光分析测定。在上臂皮肤中肥大细胞计数、血管计数和组胺含量之间发现了统计学上显著的相关性，但在前臂中没有发现类似的相关性。这两个部位的解剖差异可能是造成这种差异的原因。在同一活检样本的不同切片中发现肥大细胞计数和血管密度的巨大差异，这证实了真皮肥大细胞分布不均匀的观点。肥大细胞计数的方差分析表明，来自同一活检样本的不同切片之间的差异大于相邻活检之间的差异。在同一受试者中，相距仅2厘米的活检样本之间的组胺含量也有显著差异。
Mast cell population density was determined in normal skin from 2 regions of the arm of several healthy men and compared with blood vessel density and histamine concentration in the same sites. Mast cell and blood vessel counts were made in 1-1.5 .mu.m thick plastic sections, by light microscopy and tissue-histamine concentrations were determined by automated fluorimetric analysis. Statistically significant correlations were found between mast cell counts, blood vessel counts and histamine content in skin from the upper arm but no similar correlations were obtained in the forearm. Anatomical differences between the 2 sites may be the cause of this discrepancy. Wide variations in mast cell counts and blood vessel density were found in different sections from the same biopsy samples which confirms the notion that dermal mast cells are unevenly distributed. Analysis of variance of the mast cell counts showed that the variance between sections from different blocks from the same biopsy samples was greater than the variance between adjacent biopsies. There was also a marked variation in the histamine content between biopsy samples from sites only 2 cm apart in the same subject.