Detection of the toxin domoic acid from clam extracts using a portable surface plasmon resonance biosensor
Detection of the toxin domoic acid from clam extracts using a portable surface plasmon resonance biosensor
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DOI：
10.1016/j.hal.2006.08.001
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发表时间：
2007-02-01
期刊：
影响因子：
6.6
通讯作者：
Furlong, Clement E.
中科院分区：
文献类型：
--
作者：
Stevens, Richard C.;Soelberg, Scott D.;Furlong, Clement E.
遗忘症贝类毒素软骨藻酸是由拟菱形藻属的海洋藻类产生的。我们研制了一种便携式表面等离子体共振(SPR)生物传感器系统，用于软骨藻酸的检测。由于对贝类的软骨藻酸污染的担忧，有必要对贝类和海水中的毒素水平进行现场快速量化。制备了软骨藻酸抗体，并进行了亲和纯化。这些抗体被用来开发基于竞争和置换的分析，使用我们实验室开发的便携式六通道SPR系统。用基于竞争的SPR分析方法分析磷酸盐缓冲盐水和稀释的文蛤提取物中软骨藻酸的检测标准曲线表明，检测限为3 ppb(10 NM)，可定量范围为4至60 ppb(13-200 NM)。用标准的高效液相色谱分析方法和基于SPR的分析方法对含有中到高水平软骨藻酸的太平洋剃须刀蚌(Siliqua Patula)中软骨藻酸水平的分析结果进行了比较，两者具有很好的相关性。这项同样的技术也可用于检测浓缩藻类提取物中的软骨藻酸或海水中的高浓度软骨藻酸。(C)2006年，爱思唯尔出版。
The amnesic shellfish poison domoic acid is produced by marine algae of the genus Pseudo-nitzschia. We have developed a portable surface plasmon resonance (SPR) biosensor system for the detection of domoic acid. Because of concerns with domoic acid contamination of shellfish, there is a need for rapid field quantification of toxin levels in both shellfish and seawater. Antibodies were raised against domoic acid and affinity purified. These antibodies were used to develop competition- and displacement-based assays using a portable six-channel SPR system developed in our laboratories. Standard curves for detection of domoic acid in phosphate buffered saline and in diluted clam extracts analyzed by the competition-based SPR assay demonstrated a limit of detection of 3 ppb (10 nM) and a quantifiable range from 4 to 60 ppb (13-200 nM). Comparison of analyses for domoic acid levels in Pacific razor clams, Siliqua patula, containing moderate to high levels of domoic acid by the standard HPLC analysis protocol and the SPR-based assay gave an excellent correlation. This same technology should also function for detection of domoic acid in concentrated algal extracts or high dissolved levels in seawater. (c) 2006 Published by Elsevier B.V.