Effect of type 1 piliation on in vitro killing of Escherichia coli by mouse peritoneal macrophages.
Effect of type 1 piliation on in vitro killing of Escherichia coli by mouse peritoneal macrophages.
复制标题
1 型毛毛作用对小鼠腹腔巨噬细胞体外杀灭大肠杆菌的影响。
DOI：
10.1128/iai.58.10.3448-3454.1990
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发表时间：
1990
影响因子：
3.1
通讯作者：
Orndorff,PE
中科院分区：
文献类型：
--
作者：
Keith,BR;Harris,SL;Russell,PW;Orndorff,PE
具有影响 1 型菌毛产生、受体结合或长度的确定损伤的大肠杆菌 K-12 突变体在体外检查了其抵抗小鼠腹膜巨噬细胞杀伤的能力。突变体在含有巨噬细胞的孔中以已知的比例成对混合，孵育后，测定幸存者的比例。 1 型绒毛细胞和同基因非绒毛细胞之间的吞噬杀伤差异显着，绒毛细胞的抵抗力大约高出三倍。菌毛长度对生存影响不大，因为长菌毛突变体并不比正常长度的亲本更能抵抗杀戮。有趣的是，1 型菌毛的受体结合功能在影响抗性方面最为重要，因为缺乏结合受体能力的突变体与非菌毛突变体一样被有效地杀死。这些数据与菌毛实际上阻碍巨噬细胞杀伤而不是作为吸收的被动物理障碍的概念是一致的。
Escherichia coli K-12 mutants possessing defined lesions affecting type 1 pilus production, receptor binding, or length were examined for their ability to resist killing by mouse peritoneal macrophages in vitro. Mutants were mixed pairwise at known ratios in wells containing macrophages, and after incubation, the ratio of the survivors was assayed. The difference in phagocytic killing between type 1 piliated cells and isogenic nonpiliated cells was significant, the piliated cells being approximately threefold more resistant. Pilus length had little effect upon survival, as the long-piliated mutants were no more resistant to killing than the normal-length parents. Interestingly, the receptor-binding function of type 1 pili was most important in effecting resistance, as mutants lacking the ability to bind receptor were killed as effectively as nonpiliated mutants. These data are consistent with the notion that pili actually impede killing by macrophages rather than serve as passive physical barriers to uptake.