Isolation of Specialized Transducing Bacteriophages for Gluconate 6-Phosphate Dehydrogenase (gnd) of Escherichia coli
Isolation of Specialized Transducing Bacteriophages for Gluconate 6-Phosphate Dehydrogenase (gnd) of Escherichia coli
复制标题
大肠杆菌葡萄糖酸 6-磷酸脱氢酶 (gnd) 专用转导噬菌体的分离
DOI：
10.1128/jb.117.2.468-476.1974
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发表时间：
1974
影响因子：
3.2
通讯作者：
D. Fraenkel
中科院分区：
文献类型：
--
作者：
R. E. Wolf;D. Fraenkel
葡萄糖酸6-磷酸脱氢酶(GND)是大肠杆菌中的一种构成酶，已经用前面描述的其他基因的方法分离出了专门的转导噬菌体。GND-His区由一个F‘Episome区携带，首先被转位到TonB。通过转导，从这些菌株的φ-80溶原菌中筛选出罕见的携带GnD的噬菌体，其中1个噬菌体还携带组氨酸(φ-80gndHis)。从转导子获得高频转导裂解产物；低复数感染然后产生有缺陷的溶原。对φ80dgndHis溶原体的TonB缺失分析表明，噬菌体中的基因顺序为imm80…hisOGD...GND；根据标记挽救实验，大多数噬菌体晚期基因已被细菌脱氧核糖核酸取代。制备了一种热致裂解缺陷的λ-φ80杂化衍生物φ80dgndHis。
Specialized transducing phages for gluconate 6-phosphate dehydrogenase (gnd), a constitutive enzyme in Escherichia coli, have been isolated using a method previously described for other genes. The gnd-his region, carried on an F′ episome, was first transposed to tonB. Rare phages carrying gnd were selected, by transduction, from φ80 lysogens of these strains; one phage also carried his (φ80gndhis). From the transductants, high-frequency transducing lysates were obtained; low multiplicity of infection then yielded defective lysogens. tonB deletion analysis of the φ80dgndhis lysogen shows the order of genes in the prophage to be imm80...hisOGD...gnd; according to a marker rescue experiment most phage late genes have been replaced by bacterial deoxyribonucleic acid. A heat-inducible, lysis-defective λ-φ80 hybrid derivative of φ80dgndhis has been prepared.