Neuroregenerative effects of preinjury FK-506 administration.
Neuroregenerative effects of preinjury FK-506 administration.
复制标题
损伤前 FK-506 给药的神经再生作用。
DOI：
10.1097/01.prs.0000227628.43867.5b
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发表时间：
2006
影响因子：
3.6
通讯作者：
Mackinnon,SusanE
中科院分区：
文献类型：
--
作者：
Snyder,AlisonK;Fox,IdaK;Nichols,ChrisM;Rickman,SusanR;Hunter,DanielA;Tung,ThomasHH;Mackinnon,SusanE
背景：FK-506用于器官移植，因为它在体外促进神经突生长，并在周围神经损伤横断模型中增强神经再生。FK-506的免疫抑制机制是明确的，证明延迟给药会降低神经再生作用。本研究的目的是描述FK-506在大鼠胫神经横断injuries.Methods：8近交系雄性刘易斯大鼠每组在三个独立的组进行胫神经横断与初级修复的影响。I组给予安慰剂，II组在术前1天给予FK-506治疗，III组在术前3天给予FK-506预负荷治疗。结果：组织学和组织形态计量学结果表明，预负荷FK-506组与即刻FK-506组相比具有上级结果。两个FK-506组均上级安慰剂组。前负荷FK-506在平均总神经纤维计数（p< 0.05）、更大的神经组织百分比（p<0.05）、更大的平均神经纤维密度（p< 0.05）和更低的碎片百分比（p> 0.05）方面表现出上级的再生。平均神经纤维宽度相似的预负荷和立即FK-506组，但上级安慰剂group.Conclusion：这些数据表明，增强FK-506的神经再生效果增强神经损伤前，如进行择期手术时，管理。
Background:FK-506 is used in organ transplantation because it promotes neurite outgrowth in vitro and enhances neuroregeneration in peripheral nerve injury transection models. Immunosuppressive mechanisms of FK-506 are well defined, with demonstration of decreased neuroregenerative effects with delayed administration. The purpose of this study was to describe the effects of preinjury administration of FK-506 in rats with tibial nerve transection injury.Methods:Eight inbred male Lewis rats per group in three separate groups underwent tibial nerve transection with primary repair. Group I received placebo, group II received FK-506 treatment at 1 day before surgery, and group III received FK-506 preloading 3 days before surgery.Results:Histologic and histomorphometric results demonstrated the preload FK-506 group had superior results compared with the immediate FK-506 group. Both FK-506 groups were superior to the placebo group. The preload FK-506 demonstrated superior regeneration in mean total nerve fiber counts (p< 0.05), greater percentage neural tissue (p< 0.05), greater mean nerve fiber density (p< 0.05), and lower percentage of debris (p> 0.05). Mean nerve fiber widths were similar in the preload and immediate FK-506 groups but superior to the placebo group.Conclusion:These data suggest that enhancement of FK-506’s neuroregenerative effect is enhanced when administered before nerve injury such as when performing elective surgery.