Immunohistochemical localization of Na+/Ca2+ exchanger in human retina and retinal pigment epithelium.
Immunohistochemical localization of Na+/Ca2+ exchanger in human retina and retinal pigment epithelium.
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人视网膜和视网膜色素上皮中 Na /Ca2 交换器的免疫组织化学定位。
DOI：
10.1007/s004170050182
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Mangini,NJ
中科院分区：
文献类型：
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作者：
Loeffler,KU;Mangini,NJ
·背景：钙（Ca2+）代谢受损与多种眼部疾病的发病机制有关，这表明视网膜Ca2+稳态的调节对正常功能具有重要意义。有一些已知的转运蛋白家族可以催化Ca2+在质膜上的净外排，其中之一是Na+/Ca2+交换器。利用免疫组织化学，我们研究了人视网膜和视网膜色素上皮（RPE）中所谓的心脏型Na+/Ca2+交换剂的存在和分布。·方法：根据ABC法，用针对犬心肌肌层Na+/Ca2+交换剂产生的多克隆抗体（π）孵育10只因各种疾病过程而去核的眼睛的石蜡切片。用氨基乙基咔唑对反应进行可视化观察。·结果：抗na +/Ca2+交换剂在视网膜和RPE中均有阳性反应，但不同标本的标记不同。在神经视网膜中，穆勒细胞、内核层细胞和锥体内节染色最强烈。神经节细胞、神经纤维、外核层和杆内节段的免疫反应性不明显。外节大部分呈阴性。在RPE中，存在阳性染色，但染色强度在标本内和标本间均有差异。反应性RPE细胞显示出最强烈的标记。·结论：心脏型Na+/Ca2+交换体存在于人视网膜和RPE中。不同标本之间免疫反应性的差异可能反映了这些眼睛的不同疾病及其不同的代谢状态。某些疾病与Na+/Ca2+交换器功能障碍之间的特定关系可能对治疗方案产生重大影响。
· Background: Impaired calcium (Ca2+) metabolism has been implicated in the pathogenesis of various ocular diseases, suggesting that regulation of Ca2+homeostasis in the retina is of major significance for normal function. There are known families of transport proteins that can catalyze net Ca2+efflux across the plasma membrane, one of which is the Na+/Ca2+exchanger. Using immunohistochemistry, we have investigated the human retina and retinal pigment epithelium (RPE) for the presence and distribution of the so-called cardiac-type Na+/Ca2+exchanger. · Methods: Paraffin sections of ten eyes enucleated for various disease processes were incubated according to the ABC method with a polyclonal antibody (π) produced against the canine cardiac sarcolemmal Na+/Ca2+exchanger. The reaction was visualized with aminoethylcarbazole. · Results: There was a positive reaction with anti-Na+/Ca2+exchanger in the retina and RPE in all eyes, but the labeling varied among the different specimens. In neural retina, staining was most intense in Mueller cells, in cells of the inner nuclear layer, and in cone inner segments. Immunoreactivity was less pronounced in ganglion cells, nerve fibers, the outer nuclear layer and in rod inner segments. Outer segments appeared mostly negative. In the RPE, positive staining was present but the intensity of staining varied both within and among the specimens. Reactive RPE cells revealed the most intense labeling. · Conclusion: Na+/Ca2+exchanger of the cardiac type is present in human retina and RPE. The variation in immunoreactivity among the different specimens may reflect the different diseases of these eyes and their different metabolic states. A specific relation between certain diseases and malfunction of the Na+/Ca2+exchanger could have a major impact on therapeutic regimens.