Cathepsin-L is involved in degradation of fat body and programmed cell death in Bombyx mori
Cathepsin-L is involved in degradation of fat body and programmed cell death in Bombyx mori
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组织蛋白酶-L 参与家蚕脂肪体降解和程序性细胞死亡
DOI：
10.1016/j.gene.2020.144998
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发表时间：
2020
期刊：
影响因子：
3.5
通讯作者：
Hou Yong
中科院分区：
文献类型：
--
作者：
Yang Huan;Zhang Renze;Zhang Yuhao;Liu Qing;Li Yi;Gong Jing;Hou Yong
全变态昆虫的生活史涉及不同的阶段，组织蛋白酶在昆虫变态中发挥着重要作用。本研究探讨了家蚕组织蛋白酶L（Bm-CatL）在变态过程中的功能，并分析了其在脂肪体程序性细胞死亡（PCD）中的作用。结果表明，RNA干扰技术敲低Bm-CatL基因可导致变态期的化蛹异常和脂肪体降解延迟。此外，在下调Bm-CatL后，在脂肪体中观察到PCD抑制。为了证实这一发现，PCD诱导的家蚕胚胎（BmE）细胞的紫外线照射。我们发现Bm-CatL基因敲除后BmE细胞的PCD减弱。此外，Bm-CatL在细胞中的过表达促进PCD。总体而言，我们的结果表明，Bm-CatL参与内部组织的降解，并促进参与蛹化的细胞的PCD。因此，本研究为我们更好地了解组织蛋白酶-L在变态过程中的功能提供了依据。
The life cycle of holometabolous insects involves different stages and cathepsin plays an important role in insect metamorphosis. In the present study, we investigated the function of Bombyx mori cathepsin-L (Bm-CatL) during metamorphosis and analyzed their role in programmed cell death (PCD) of the fat body. The results showed that knockdown of Bm-CatL by RNA interference led to abnormal pupation and a delay in fat body degradation during metamorphosis. Furthermore, PCD inhibition was observed in the fat body after downregulation of Bm-CatL. To confirm this finding, PCD was induced in Bombyx mori embryonic (BmE) cells by ultraviolet ray irradiation. We found that the PCD of BmE cells was weakened after knocking down Bm-CatL. Moreover, overexpression of Bm-CatL in cells promoted PCD. Overall, our results showed that Bm-CatL is involved in the degradation of internal tissues and promotes the PCD of cells involved in the pupation of silkworms. Thus, this study provides us with a better understanding for function of cathepsin-L during metamorphosis.