RNA-Seq Reveals Enhanced Sugar Metabolism in Streptococcus mutans Co-cultured with Candida albicans within Mixed-Species Biofilms.
RNA-Seq Reveals Enhanced Sugar Metabolism in Streptococcus mutans Co-cultured with Candida albicans within Mixed-Species Biofilms.
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DOI：
10.3389/fmicb.2017.01036
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发表时间：
2017
影响因子：
5.2
通讯作者：
Koo H
中科院分区：
文献类型：
--
作者：
He J;Kim D;Zhou X;Ahn SJ;Burne RA;Richards VP;Koo H
幼儿龋齿（ECC）是影响全世界儿童的最流行的传染病之一，其可导致猖獗的龋齿，其是痛苦的且治疗昂贵的。以往的研究支持变形链球菌和白色念珠菌之间的相互作用与ECC的发病机制有关。假丝酵母的存在增强了S.变形菌的生长，健身和积累内的生物膜在体外，虽然这些行为的分子基础是不确定的。利用已建立的共培养生物膜模型和RNA-Seq技术，我们研究了C. albicans影响S.变异人C.白色念珠菌显著改变了S.双菌种生物膜中的变异链球菌，导致393个基因的差异表达，相比单菌种生物膜的S。变异体通过基因本体分析，大多数上调基因与碳水化合物转运和代谢/分解代谢过程有关。KEGG途径影响分析显示丙酮酸和半乳糖代谢升高，表明与C。白色念珠菌影响S.变异体代谢物的分析证实了碳水化合物代谢的增加，在共培养的生物膜的培养基中甲酸盐的量升高。此外，与C. albicans改变了S.与适应性和毒力相关的变形菌信号转导（comC和ciaRH）基因。有趣的是，突变素（细菌素）和CRISPR基因的表达被下调。总的来说，这些数据提供了一个全面的洞察S。C.白色念珠菌，并提供新的见解如何细菌-真菌的相互作用可能会增加龋齿的严重性。
Early childhood caries (ECC), which can lead to rampant tooth-decay that is painful and costly to treat, is one of the most prevalent infectious diseases affecting children worldwide. Previous studies support that interactions between Streptococcus mutans and Candida albicans are associated with the pathogenesis of ECC. The presence of Candida enhances S. mutans growth, fitness and accumulation within biofilms in vitro, although the molecular basis for these behaviors is undefined. Using an established co-cultivation biofilm model and RNA-Seq, we investigated how C. albicans influences the transcriptome of S. mutans. The presence of C. albicans dramatically altered gene expression in S. mutans in the dual-species biofilm, resulting in 393 genes differentially expressed, compared to mono-species biofilms of S. mutans. By Gene Ontology analysis, the majority of up-regulated genes were related to carbohydrate transport and metabolic/catabolic processes. KEGG pathway impact analysis showed elevated pyruvate and galactose metabolism, suggesting that co-cultivation with C. albicans influences carbohydrate utilization by S. mutans. Analysis of metabolites confirmed the increases in carbohydrate metabolism, with elevated amounts of formate in the culture medium of co-cultured biofilms. Moreover, co-cultivation with C. albicans altered transcription of S. mutans signal transduction (comC and ciaRH) genes associated with fitness and virulence. Interestingly, the expression of genes for mutacins (bacteriocins) and CRISPR were down-regulated. Collectively, the data provide a comprehensive insight into S. mutans transcriptomic changes induced by C. albicans, and offer novel insights into how bacterial–fungal interactions may enhance the severity of dental caries.