VP19 is important for the envelope coating of white spot syndrome virus
VP19 is important for the envelope coating of white spot syndrome virus
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VP19对于白斑综合症病毒的包膜涂层很重要
DOI：
10.1016/j.virusres.2019.197666
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发表时间：
2019-09-01
期刊：
影响因子：
5
通讯作者：
Li, Fang
中科院分区：
文献类型：
--
作者：
Li, Li;Hong, Yongcong;Li, Fang
VP19 是白斑综合症病毒 (WSSV) 的主要包膜蛋白，WSSV 是虾扇形虾的重要病原体。然而，VP19 在 WSSV 组装和感染中的确切功能尚不清楚。为了了解 VP19 的功能，通过 RNA 干扰将该基因敲低。我们发现，vp19 基因特异的 dsRNA 显着减少了受感染动物中 WSSV 基因组 DNA 的复制。透射电子显微镜的进一步研究表明，抑制 VP19 可以阻止子代病毒粒子的包膜涂层，导致宿主细胞中产生大量没有外层（包膜）的未成熟病毒颗粒。 SDS-PAGE 分析进一步证实了这一发现，该分析显示未正确组装的病毒体中 VP19 和其他包膜蛋白的丢失。这些结果表明 VP19 对于 WSSV 包膜涂层至关重要。
VP19 is a major envelope protein of white spot syndrome virus (WSSV), an important pathogen of fanned shrimp. However, the exact function of VP19 in WSSV assembly and infection is unknown. To understand the function of VP19, the gene was knocked down by RNA interference. We found that the dsRNA specific for vp19 gene dramatically reduced the replication of WSSV genomic DNA in infected animals. Further investigation by transmission electron microscopy showed that inhibition of VP19 prevented envelope coating of progeny virions, resulting in a high amount of immature virus particles without outer layer (envelope) in the host cells. This finding was further confirmed by SDS-PAGE analysis, which showed the loss of VP19 and other envelope proteins from the improperly assembled virions. These results suggest that VP19 is essential for WSSV envelope coating.