Stage-specific expression of proprotein form of hyaluronan binding protein 1 (HABP1) during spermatogenesis in rat
Stage-specific expression of proprotein form of hyaluronan binding protein 1 (HABP1) during spermatogenesis in rat
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DOI：
10.1002/mrd.10135
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发表时间：
2002-06-01
影响因子：
2.5
通讯作者：
Datta, K
中科院分区：
文献类型：
--
作者：
Bharadwaj, A;Ghosh, I;Datta, K
34 kda透明质酸结合蛋白1 （HABP1）在精子表面的存在及其在受精中的作用已经被证实（Ranganathan et al.， 1994: Mol生殖发育38:6976）。在本通讯中，我们检测了HABP1在成年大鼠睾丸中精子发生过程中的表达。有趣的是，使用抗rhabp1抗体，我们检测到一种55 kDa的蛋白，该蛋白仅存在于睾丸中，而不存在于脾脏和肝脏等其他体细胞组织中。然而，即使在睾丸中，也只观察到一个1.63 kb的HABP1 mRNA转录本。此外，我们利用针对HABP1蛋白区存在的十肽的抗体，证实了这种睾丸特异性55 kDa蛋白在免疫学上与HABP1的蛋白形式相同。使用这两种抗体对睾丸、脾脏和肝脏组织进行比较免疫组化，支持HABP1蛋白形式仅存在于睾丸的观察。通过RT-PCR产物推测，睾丸中HABP1 mRNA的表达高于肝脏和脾脏。最后，对精小管免疫组化染色的详细研究显示，HABP1蛋白在粗线精母细胞和圆形精母细胞等生殖细胞的特定阶段表达，而在细长精母细胞中不表达，提示HABP1蛋白可能在生精分化中起作用。(C) 2002 Wiley-Liss, Inc。
The presence of the 34-kDa hyaluronan binding protein 1 (HABP1) on sperm surface and its role in fertilization is already established (Ranganathan et al., 1994: Mol Reprod Dev 38:6976). In the present communication, we examined the expression of HABP1 in adult rat testis during spermatogenesis. Interestingly, using anti-rHABP1 antibody, we detected a protein of 55 kDa which was present only in testis, but not in other somatic tissues like spleen and liver. However, even in testis, only one transcript of HABP1 mRNA of 1.63 kb was observed. In addition, we confirm that this testis-specific 55 kDa protein was immunologically identical with proprotein form of HABP1 using antibody raised against a decapeptide present in the proprotein region of HABP1. Comparative immunohistochemistry of testis, spleen, and liver tissues using both the antibodies supported the observation that the proprotein form of HABP1 is present only in testis. Higher mRNA expression of HABP1 in testis as compared to that of liver and spleen could be speculated from the RT-PCR product, Finally, detailed study of the immunohistochemical staining of the seminiferous tubules revealed the expression of the HABP1 proprotein in specific stages of germ cells, like pachytene spermatocytes and round spermatids, but not in elongated ones, suggesting a possible role of HABP1 proprotein in spermatogenic differentiation. (C) 2002 Wiley-Liss, Inc.