COMPLETE DECHLORINATION OF TETRACHLOROHYDROQUINONE BY CELL-EXTRACTS OF PENTACHLOROPHENOL-INDUCED RHODOCOCCUS-CHLOROPHENOLICUS
COMPLETE DECHLORINATION OF TETRACHLOROHYDROQUINONE BY CELL-EXTRACTS OF PENTACHLOROPHENOL-INDUCED RHODOCOCCUS-CHLOROPHENOLICUS
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DOI：
10.1128/jb.169.11.5125-5130.1987
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发表时间：
1987-11-01
影响因子：
3.2
通讯作者：
SALKINOJASALONEN, MS
中科院分区：
文献类型：
--
作者：
APAJALAHTI, JHA;SALKINOJASALONEN, MS
本文描述了氯酚红球菌诱导酶催化四氯对苯二酚合成1，2，4-三苯二酚的反应过程。四氯对苯二酚首先在包括水解和还原脱氯的反应中转化为二氯三苯酚；没有检测到三氯化中间体。二氯三酚随后被还原脱氯为一氯三酚，最后被还原为1，2，4-三酚。细胞提取物还以较低的速率催化三氯对苯二酚的还原脱氯，主要是生成2，3-二氯对苯二酚。据我们所知，这是第一次演示了细菌酶对芳香族的还原脱氯。
In this paper we describe the sequence of reactions leading from tetrachloro-para-hydroquinone to 1,2,4-trihydroxybenzene by inducible enzymes of Rhodococcus chlorophenolicus. Tetrachlorohydroquinone was first converted to a dichlorotrihydroxybenzene in a reaction involving both hydrolytic and reductive dechlorination; no trichlorinated intermediate was detected. Dichlorotrihydroxybenzene was subsequently reductively dechlorinated to a monochlorotrihydroxybenzene and finally to 1,2,4-trihydroxybenzene. The cell extract also catalyzed, at a lower rate, reductive dechlorination of trichlorohydroquinone, mainly to 2,3-dichlorohydroquinone. To our knowledge this is the first demonstration of reductive aromatic dechlorination by bacterial enzymes.